Home LiteratureArticle Details
PMID: 4922290 Published · ppublish English Journal Article

Structure and function of bacterial ribosomes. XI. Dependence of 50S ribosomal assembly on simultaneous assembly of 30S subunits.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 3 ·1970-11-00 ·Pages 1440-7

Nashimoto H, Nomura M

Abstract

Some spectinomycin-resistant mutants of Escherichia coli are cold-sensitive. They are unable to assemble both 30S and 50S ribosomal subunits at low temperatures. They accumulate two kinds of incomplete particles, related to 30S and 50S subunits respectively. A single mutation, causing an alteration in a 30S ribosomal component, is responsible for these phenotypes. These results show that assembly of 50S subunits in vivo is dependent on simultaneous assembly of 30S subunits. On the other hand, the assembly of 30S subunits appears to be independent of 50S assembly.

MeSH Terms
Bacterial Proteins/biosynthesis Centrifugation, Density Gradient Drug Resistance, Microbial Erythromycin/pharmacology Escherichia coli Genetics, Microbial Immunogenetics Mutation Phenotype Ribosomes/analysis Streptomycin/pharmacology Transduction, Genetic
Chemicals
Bacterial Proteins Erythromycin Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nashimoto H
Nomura M
References (20)
20 references, click to expand
  1. A ribosomal ambiguity mutation.
    J Mol Biol. 1969 Jan 14;39(1):95-112 PMID: 4938819
  2. Cold-sensitive mutations in Salmonella typhimurium which affect ribosome synthesis.
    J Bacteriol. 1969 Mar;97(3):1298-304 PMID: 4887510
  3. Antibiotic inhibitors of the bacterial ribosome.
    Bacteriol Rev. 1968 Dec;32(4 Pt 2):493-528 PMID: 4179192
  4. Altered ribosomal protein in streptomycin-dependent Escherichia coli.
    Science. 1969 Dec 5;166(3910):1282-4 PMID: 4900367
  5. Sensitivity and Resistance to Spectinomycin in Escherichia coli.
    J Bacteriol. 1969 Nov;100(2):939-47 PMID: 16559073
  6. Effect of spectinomycin on polypeptide synthesis in extracts of Escherichia coli.
    J Mol Biol. 1967 Oct 14;29(1):203-15 PMID: 4228335
  7. SPONTANEOUS MUTATION TO STREPTOMYCIN RESISTANCE AND DEPENDENCE IN ESCHERICHIA COLI.
    J Bacteriol. 1949 May;57(5):565-72 PMID: 16561734
  8. Ribosomal protein conferring sensitivity to the antibiotic spectinomycin in Escherichia coli.
    Science. 1968 Jul 4;165(3888):85-6 PMID: 4892072
  9. Genetic studies of the ribosomal proteins in Escherichia coli. IV. Pattern of the alteration of ribosomal protein components in mutants resistant to spectinomycin or erythromycin in different strains of Escherichia coli.
    Mol Gen Genet. 1970;107(1):39-49 PMID: 4249314
  10. Synthesis and maturation of ribosomal RNA in Escherichia coli.
    J Mol Biol. 1969 Dec 14;46(2):281-303 PMID: 4902519
  11. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
    Biochemistry. 1967 Jun;6(6):1818-27 PMID: 6035921
  12. Unique protein moieties for 30S and 50S ribosomes of Escherichia coli.
    J Bacteriol. 1969 Jun;98(3):1080-6 PMID: 4892365
  13. Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):384-91 PMID: 4895536
  14. Mapping of the galactose genes of Escherichia coli by transduction with phage P1.
    Virology. 1963 Feb;19:117-26 PMID: 14011089
  15. Escherichia coli: reversion from streptomycin dependence, a mutation in a specific 30 s ribosomal protein.
    J Mol Biol. 1969 Jul 28;43(2):327-9 PMID: 4896375
  16. Ribosomal proteins of E. Coli: stoichiometry and implications for ribosome structure.
    Cold Spring Harb Symp Quant Biol. 1969;34:25-38 PMID: 4985888
  17. Separation of bacterial ribosomal ribonucleic acid from its macromolecular precursors by polyacrylamide gel electrophoresis.
    J Bacteriol. 1968 Mar;95(3):986-90 PMID: 4966836
  18. Studies on the assembly of ribosomes in vivo.
    Cold Spring Harb Symp Quant Biol. 1969;34:69-75 PMID: 4909525
  19. Hybrid 30S ribosomal particles reconstituted from components of different bacterial origins.
    Nature. 1968 Aug 24;219(5156):793-9 PMID: 4876933
  20. STREPTOMYCIN ACTION AND THE RIBOSOME.
    Proc Natl Acad Sci U S A. 1964 Apr;51:703-9 PMID: 14166778
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.3.1440
Published
1970-11-00
Pages
1440-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283371
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.3.1440 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2