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PMID: 6087279 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Composite human VK genes and a model of their evolution.

Nucleic acids research ·Vol. 12 ·No. 13 ·1984-07-11 ·Pages 5249-63

Jaenichen HR, Pech M, Lindenmaier W, Wildgruber N, Zachau HG

Abstract

A phage library and two cosmid libraries were screened for human VK genes. Two recombinant phage and four cosmid clones were analysed in detail by restriction mapping and sequencing. Each one contained a single VKI sequence. Two of these six sequences are potentially functional VK genes and four are pseudogenes. Two pseudogenes derived from different genomic DNAs are highly homologous and are therefore either allelic variants or the products of a recent duplication event. Comparisons of our sequences with all fully determined human VKI amino acid and DNA sequences reveal identical segments which at first sight appear like minigenes. But these segments do not coincide with the subregions and some of the segments include both, framework and complementarity determining regions (FR, CDR, ref. 2). The findings may be explained by an evolutionary model generating composite genes by gene conversion and selection.

MeSH Terms
Alleles Amino Acid Sequence Base Sequence Biological Evolution Cloning, Molecular DNA Restriction Enzymes Genes Humans Immunoglobulin Light Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin kappa-Chains/genetics Plasmids
Chemicals
Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jaenichen H R
Pech M
Lindenmaier W
Wildgruber N
Zachau H G
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.13.5249
Published
1984-07-11
Pages
5249-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318917
Subset
IM
Databases
GENBANK
M23848, M23849, M23850, M23851, M23852, M23853
External Links
PubMed source DOI: 10.1093/nar/12.13.5249 PMC Full Text View journal details
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