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PMID: 6093049 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the repressor gene of the RA1 tetracycline resistance determinant: structural and functional comparison with three related Tet repressor genes.

Nucleic acids research ·Vol. 12 ·No. 20 ·1984-10-25 ·Pages 7693-703

Unger B, Klock G, Hillen W

Abstract

The tetracycline resistance determinant of RA1 was cloned. It consists of at least two genes oriented with opposite polarity, tetA for resistance and tetR for regulation. The transcriptional control sequence was identified and analyzed. It consists of overlapping promotors with divergent orientation and a tandem arrangement of operators. Nucleotide sequencing revealed two open reading frames. One codes for a protein which was identified as a Tet repressor by comparing its primary structure with those of other Tet repressors. The RA1 tetR gene codes for 218 amino acids with a calculated molecular weight of 24.4 kDa. In the primary sequence of the RA1-, pSC101-, Tn10-, and RP1/Tn1721-encoded Tet repressors, 36% of the amino acids are identical. This homology is clustered within the first 150 amino acids, 49% of which are identical among all four proteins. These results are discussed with respect to their structure and function in comparison to other DNA binding proteins.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Drug Resistance, Microbial Escherichia coli/drug effects,genetics Genes Genes, Bacterial Genes, Regulator Tetracycline/pharmacology Transcription, Genetic
Chemicals
DNA Restriction Enzymes Tetracycline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Unger B
Klock G
Hillen W
References (22)
22 references, click to expand
  1. Characterization of a mini-ColC1 plasmid.
    J Bacteriol. 1976 Apr;126(1):447-53 PMID: 770430
  2. Prediction of the secondary structure of proteins from their amino acid sequence.
    Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 PMID: 364941
  3. Construction and mapping of recombinant plasmids used for the preparation of DNA fragments containing the Escherichia coli lactose operator and promoter.
    J Biol Chem. 1979 Jun 25;254(12):5527-34 PMID: 376510
  4. Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10.
    J Bacteriol. 1979 Jun;138(3):705-14 PMID: 378932
  5. Identification by deletion analysis of an inducible protein required for plasmid pSC101-mediated tetracycline resistance.
    Plasmid. 1979 Jul;2(3):417-25 PMID: 384420
  6. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  7. Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type.
    Nucleic Acids Res. 1980 May 10;8(9):1893-912 PMID: 6159596
  8. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974-7 PMID: 7001450
  9. The tetracyclines: prospects at the beginning of the 1980s.
    J Antimicrob Chemother. 1981 Jul;8(1):5-21 PMID: 7019198
  10. pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.
    Nucleic Acids Res. 1982 Oct 11;10(19):5765-72 PMID: 6292850
  11. Tn1721-encoded tetracycline resistance: mapping of structural and regulatory genes mediating resistance.
    J Bacteriol. 1983 Jan;153(1):116-23 PMID: 6294046
  12. Revised sequence of the tetracycline-resistance gene of pBR322.
    Gene. 1983 May-Jun;22(2-3):277-80 PMID: 6307828
  13. The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis.
    Nucleic Acids Res. 1983 Sep 10;11(17):6089-105 PMID: 6310527
  14. Overlapping divergent promoters control expression of Tn10 tetracycline resistance.
    Gene. 1983 Aug;23(2):149-56 PMID: 6311683
  15. Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions.
    J Mol Biol. 1983 Sep 25;169(3):707-21 PMID: 6313933
  16. Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10.
    J Bacteriol. 1983 Dec;156(3):1188-91 PMID: 6315680
  17. Frequency of tetracycline resistance determinant classes among lactose-fermenting coliforms.
    Antimicrob Agents Chemother. 1983 Dec;24(6):835-40 PMID: 6660856
  18. Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322.
    Gene. 1983 Nov;25(1):83-92 PMID: 6319234
  19. Control of expression of the Tn10-encoded tetracycline resistance operon. II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region.
    J Mol Biol. 1984 Jan 15;172(2):185-201 PMID: 6229640
  20. Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant.
    Nucleic Acids Res. 1984 Jun 25;12(12):4849-63 PMID: 6330687
  21. Atypical deletions generated by mutated IS102 elements.
    Mol Gen Genet. 1984;195(3):452-8 PMID: 6088945
  22. Heterogeneity of tetracycline resistance determinants.
    Plasmid. 1980 Mar;3(2):99-108 PMID: 6927764
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.20.7693
Published
1984-10-25
Pages
7693-703
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320194
Subset
IM
Databases
GENBANK
X01083
External Links
PubMed source DOI: 10.1093/nar/12.20.7693 PMC Full Text View journal details
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