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PMID: 6096669 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and analysis of pif, replication and leading regions of the F plasmid.

Molecular & general genetics : MGG ·Vol. 197 ·No. 1 ·1984-00-00 ·Pages 129-36

Jackson R, Cram D, Ray A, DiBerardino D, Skurray R

Abstract

We describe the molecular cloning of BglII fragments of the hybrid plasmid pRS5 (pSC101 and EcoRI fragments of F; f7, f5, f3 and f6). The clones isolated were examined for the expression of F-specified replication, incompatibility, mobilization and inhibition of T7 bacteriophage multiplication. Proteins directed by the BglII clones were labelled in Escherichia coli K12 maxicells and analyzed by SDS-polyacrylamide gel electrophoresis. The sizes of previously reported proteins, encoded by the replication, incompatibility and leading regions encompassed by these plasmids have been confirmed in this study. In addition, the results demonstrate that a pif gene, which encodes an 80,000 dalton polypeptide essential for the inhibition T7 phage multiplication, is located on the BglII fragment that spans the junction of EcoRI fragments f7 and f5.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics F Factor Genes Genes, Bacterial T-Phages/physiology
Chemicals
Bacterial Proteins DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jackson R
Cram D
Ray A
DiBerardino D
Skurray R
References (40)
40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327933
Published
1984-00-00
Pages
129-36
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327933
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