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PMID: 6185422 Published · ppublish English Journal Article

Mapping of a gene in Vibrio cholerae that determines the antigenic structure of cholera toxin.

Infection and immunity ·Vol. 38 ·No. 3 ·1982-12-00 ·Pages 1109-16

Saunders DW, Schanbacher KJ, Bramucci MG

Abstract

In Ouchterlony-type immunodiffusion gels, the cholera toxin produced by the classical Vibrio cholerae strain 569B was indistinguishable from the cholera toxin of the Eltor strain RJ1. However, the cholera toxin produced by both of these strains was incompletely cross-reactive with the cholera toxin produced by the Eltor strain 3083-2. The allele of the gene responsible for the 569B and RJ1 type of toxin was designated vct-1, and the allele responsible for the 3083-2 type of toxin was designated vct-2. The vct-1 allele was transferred from RJ1 donors to 3083-2 recipients by conjugation. The vct locus was found to be between met and trp on the V. cholerae linkage map.

MeSH Terms
Alleles Antigens, Bacterial/genetics Cholera Toxin/genetics,immunology Chromosome Mapping Chromosomes, Bacterial Epitopes/genetics Genes Genes, Bacterial Vibrio cholerae/genetics
Chemicals
Antigens, Bacterial Epitopes Cholera Toxin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saunders D W
Schanbacher K J
Bramucci M G
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32 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.38.3.1109-1116.1982
Published
1982-12-00
Pages
1109-16
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347864
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.38.3.1109-1116.1982 PMC Full Text View journal details
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