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PMID: 6195145 Published · ppublish English Journal Article

Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF.

Journal of bacteriology ·Vol. 156 ·No. 2 ·1983-11-00 ·Pages 800-8

Jentsch S

Abstract

The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the sequence, rendering such sites resistant to MspI degradation and leaving the majority of sites sensitive to HpaII degradation.

MeSH Terms
Bacillus subtilis/genetics Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes/genetics DNA, Bacterial/genetics,isolation & purification Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific Genes Genes, Bacterial Methylation Plasmids
Chemicals
Bacterial Proteins DNA, Bacterial DNA Restriction Enzymes Deoxyribonuclease HpaII CGCG-specific type II deoxyribonucleases CTCGAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jentsch S
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46 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.2.800-808.1983
Published
1983-11-00
Pages
800-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217898
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.156.2.800-808.1983 PMC Full Text View journal details
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