Home LiteratureArticle Details
PMID: 6200884 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Anti-hypervariable region antibody induced by a defined peptide: an approach for studying the structural correlates of idiotypes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 6 ·1984-03-00 ·Pages 1784-8

Chen PP, Houghten RA, Fong S, Rhodes GH, Gilbertson TA, Vaughan JH, Lerner RA, Carson DA

Abstract

The structural correlates of idiotypes have been sought in several antibody systems. However, the precise molecular basis of idiotypes are exceedingly difficult to define. Antibodies of predetermined specificity can be induced by immunization with synthetic peptides. In the present experiments, a synthetic peptide corresponding to a hypervariable region on a monoclonal human IgM rheumatoid factor (Sie) has been used to induce specific anti-hypervariable region antibodies. The antibodies bound to the intact Ig molecule and to its isolated heavy chains but not to other IgM paraproteins nor to pooled human IgG. The binding of the antibody to the intact IgM was inhibited specifically by the free peptide, and the antibody activity was removed by a peptide-coupled affinity column. These results clearly demonstrate that specific anti-idiotypic antibody of predefined specificity can be induced by a hypervariable region peptide. Antibodies of this class may provide a new tool for defining the structural correlates of idiotypes.

MeSH Terms
Animals Antibodies, Anti-Idiotypic/immunology Antibody Formation Epitopes Immunoglobulin G/immunology Immunoglobulin Idiotypes/immunology Immunoglobulin M/immunology Immunoglobulin Variable Region/immunology Peptide Fragments/immunology Rabbits
Chemicals
Antibodies, Anti-Idiotypic Epitopes Immunoglobulin G Immunoglobulin Idiotypes Immunoglobulin M Immunoglobulin Variable Region Peptide Fragments
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Chen P P
Houghten R A
Fong S
Rhodes G H
Gilbertson T A
Vaughan J H
Lerner R A
Carson D A
References (39)
39 references, click to expand
  1. The internal image of IgG in cross-reactive anti-idiotypic antibodies against human rheumatoid factors.
    J Immunol. 1983 Aug;131(2):719-24 PMID: 6863929
  2. The T lymphocyte antigen receptor--paradigm lost.
    Nature. 1982 Dec 16;300(5893):583-8 PMID: 6755269
  3. Cross-idiotypic specificity among monoclonal IgM proteins with anti- -globulin activity.
    J Exp Med. 1973 Feb 1;137(2):331-42 PMID: 4119588
  4. Tapping the immunological repertoire to produce antibodies of predetermined specificity.
    Nature. 1982 Oct 14;299(5884):593-6 PMID: 6181415
  5. Towards a chemical definition of idiotypy.
    Fed Proc. 1977 Feb;36(2):204-6 PMID: 65295
  6. Tritiated porcine dynorphin (1-17): synthesis and characterization.
    Life Sci. 1982 Oct 18-25;31(16-17):1805-8 PMID: 6130444
  7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  8. Immunogenic structure of the influenza virus hemagglutinin.
    Cell. 1982 Mar;28(3):477-87 PMID: 6176330
  9. Towards a network theory of the immune system.
    Ann Immunol (Paris). 1974 Jan;125C(1-2):373-89 PMID: 4142565
  10. Human beta-endorphin: synthesis and characterization of analogs iodinated and tritiated at tyrosine residues 1 and 27.
    Int J Pept Protein Res. 1980 Oct;16(4):311-20 PMID: 7461911
  11. Molecular recognition and the future of monoclonal antibodies.
    Nature. 1982 Mar 18;296(5854):200-2 PMID: 7063025
  12. Antibodies that react with predetermined sites on proteins.
    Science. 1983 Feb 11;219(4585):660-6 PMID: 6186024
  13. Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity.
    J Immunol. 1982 Mar;128(3):1176-80 PMID: 6173424
  14. Antibodies with specificities to preselected protein regions evoked by free synthetic peptides representing protein antigenic sites or other surface locations: demonstration with myoglobin.
    Mol Immunol. 1983 May;20(5):567-70 PMID: 6192328
  15. The use of idiotypes as markers for antibody variable regions in the rabbit.
    Fed Proc. 1977 Feb;36(2):214-20 PMID: 65296
  16. Immunochemical analysis of the idiotypes of mouse myeloma proteins with specificity for levan or dextran.
    J Exp Med. 1974 Jan 1;139(1):137-47 PMID: 4128444
  17. Individual Antigenic Specificity of Isolated Antibodies.
    Science. 1963 Jun 14;140(3572):1218-9 PMID: 17837506
  18. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  19. Human immunoglobulin D segments encoded in tandem multigenic families.
    Nature. 1981 Dec 17;294(5842):631-5 PMID: 7312051
  20. Structure of antibodies with shared idiotypy: the complete sequence of the heavy chain variable regions of two immunoglobulin M anti-gamma globulins.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4032-6 PMID: 4139708
  21. A common idiotope on human rheumatoid factors identified by a hybridoma antibody.
    Mol Immunol. 1983 Oct;20(10):1081-7 PMID: 6201726
  22. Evolutionary aspects of immunoglobulin heavy chain variable region (VH) gene subgroups.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):855-9 PMID: 6298778
  23. Recurrent idiotopes and internal images.
    EMBO J. 1982;1(2):243-7 PMID: 6985352
  24. A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells.
    J Exp Med. 1982 Dec 1;156(6):1848-53 PMID: 7175441
  25. Recovery of binding activity in reconstituted mouse myeloma proteins.
    Biochemistry. 1971 Jun 22;10(13):2525-30 PMID: 4104270
  26. Implications of the presence of an internal image of the antigen in anti-idiotypic antibodies: possible application to vaccine production.
    Clin Immunol Immunopathol. 1981 Dec;21(3):397-406 PMID: 6173154
  27. Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6561-5 PMID: 6450418
  28. Idiotypic manipulation in mice: BALB/c mice can express the crossreactive idiotype of A/J mice.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4474-8 PMID: 6576348
  29. The amino acid sequence of the variable regions of the light chains from two idiotypically cross reactive IgM anti-gamma globulins.
    Ann Immunol (Paris). 1976 Jun-Jul;127(3-4):261-71 PMID: 60899
  30. Anti-idiotype and anti-hormone receptor antibodies.
    Springer Semin Immunopathol. 1983;6(1):67-78 PMID: 6310806
  31. Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes.
    Cell. 1981 Dec;27(3 Pt 2):583-91 PMID: 6101209
  32. T and B cells that recognize the same antigen do not transcribe similar heavy chain variable region gene segments.
    J Exp Med. 1983 Jul 1;158(1):192-209 PMID: 6190977
  33. Anti-immunoglobulin antibodies. III. Properties of sequential anti-idiotypic antibodies to heterologous anti-gamma globulins. Detection of reactivity of anti-idiotype antibodies with epitopes of Fc fragments (homobodies) and with epitopes and idiotopes (epibodies).
    J Exp Med. 1982 Oct 1;156(4):986-99 PMID: 6185609
  34. A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6984-8 PMID: 6983692
  35. New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates.
    Biochemistry. 1979 Feb 20;18(4):690-3 PMID: 84683
  36. Idiotypic regulation in immune networks.
    Contemp Top Mol Immunol. 1981;8:113-48 PMID: 7018828
  37. A monoclonal antibody that detects a V kappa-TEPC15 idiotypic determinant cross-reactive with a Thy-1 determinant.
    J Exp Med. 1981 May 1;153(5):1068-79 PMID: 6166711
  38. Amino acid sequence of homogeneous antibodies to dextran and DNA rearrangements in heavy chain V-region gene segments.
    Nature. 1980 Jan 3;283(5742):35-40 PMID: 6765983
  39. Complete amino acid sequence of variable domains from two monoclonal human anti-gamma globulins of the Wa cross-idiotypic group: suggestion that the J segments are involved in the structural correlate of the idiotype.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3799-803 PMID: 6167990
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.6.1784
Published
1984-03-00
Pages
1784-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345005
Subset
IM
Grants
NIA NIH HHS · AG04100 · United States
NIADDK NIH HHS · AM21175 · United States
NIADDK NIH HHS · AM25443 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.6.1784 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2