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PMID: 6246271 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA.

Journal of virology ·Vol. 34 ·No. 2 ·1980-05-00 ·Pages 402-9

Lusby E, Fife KH, Berns KI

Abstract

The inverted terminal repetition in adeno-associated virus type 2 DNA has been sequenced. The terminal repetition contain 145 nucleotides of which the first 125 nucleotides can self-base pair to form a T-shaped hairpin structure. Both restriction endonuclease analysis with SmaI and BglI and direct sequence analysis of the SmaI fragments provide evidence for two sequences in the region of the terminal repetition between nucleotides 44 and 81. The two sequences represent an inversion of the first 125 nucleotides of the terminal repetition. Based on these data a model for adeno-associated virus DNA replication is presented which agrees in detail with a general model for eucaryotic DNA replication originally proposed by Cavalier-Smith (T. Cavalier-Smith, Nature [London] 18:672--684, 1976).

MeSH Terms
Base Sequence DNA Replication DNA Restriction Enzymes DNA, Viral/analysis Dependovirus/analysis,metabolism Models, Biological
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lusby E
Fife K H
Berns K I
References (23)
23 references, click to expand
  1. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
    Biochem J. 1964 Jan;90(1):147-9 PMID: 5832284
  2. Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions.
    Science. 1969 Dec 5;166(3910):1280-2 PMID: 5350322
  3. Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):863-9 PMID: 5264145
  4. Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands.
    J Virol. 1970 Jun;5(6):693-9 PMID: 5429749
  5. Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains.
    J Virol. 1972 Feb;9(2):394-6 PMID: 5014934
  6. Origin of concatemeric T7 DNA.
    Nat New Biol. 1972 Oct 18;239(94):197-201 PMID: 4507727
  7. Self-complementarity of terminal sequences within plus or minus strands of adenovirus-associated virus DNA.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):215-9 PMID: 4509654
  8. Arrangement of nucleotide sequences in adeno-associated virus DNA.
    J Mol Biol. 1973 Sep 15;79(2):207-25 PMID: 4586409
  9. Letter: Visualization of the inverted terminal repetition in adeno-associated virus DNA.
    J Mol Biol. 1974 Jan 15;82(2):267-71 PMID: 4816655
  10. Study of the fine structure of adeno-associated virus DNA with bacterial restriction endonucleases.
    J Virol. 1975 Sep;16(3):712-9 PMID: 1159899
  11. Palindromic base sequences and replication of eukaryote chromosome ends.
    Nature. 1974 Aug 9;250(5466):467-70 PMID: 4469597
  12. Concatemers of alternating plus and minus strands are intermediates in adenovirus-associated virus DNA synthesis.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):742-6 PMID: 1062784
  13. Multiple structures of adeno-associated virus DNA: analysis of terminally labeled molecules with endonuclease R-Hae III.
    J Virol. 1976 May;18(2):672-84 PMID: 1271522
  14. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  15. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  16. Structure and nucleotide sequence of the terminal regions of adeno-associated virus DNA.
    Virology. 1977 May 15;78(2):475-7 PMID: 194395
  17. Origin and termination of adeno-associated virus DNA replication.
    Virology. 1977 May 15;78(2):488-99 PMID: 867815
  18. Evidence for two nucleotide sequence orientations within the terminal repetition of adeno-associated virus DNA.
    J Virol. 1977 Nov;24(2):627-34 PMID: 916029
  19. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  20. Adeno-associated virus DNA replication: nonunit-length molecules.
    Virology. 1979 Feb;93(1):57-68 PMID: 219605
  21. Structure of the 3' hairpin termini of four rodent parvovirus genomes: nucleotide sequence homology at origins of DNA replication.
    Cell. 1979 Jul;17(3):691-703 PMID: 225040
  22. Nucleotide sequence of the self-priming 3' terminus of the single-stranded DNA extracted from the parvovirus Kilham rat virus.
    J Virol. 1979 Jun;30(3):946-50 PMID: 225552
  23. ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES.
    Science. 1965 Aug 13;149(3685):754-6 PMID: 14325163
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.34.2.402-409.1980
Published
1980-05-00
Pages
402-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288718
Subset
IM
Databases
GENBANK
K01624, K01625
External Links
PubMed source DOI: 10.1128/JVI.34.2.402-409.1980 PMC Full Text View journal details
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