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PMID: 6262774 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of herpes simplex virus type I thymidine kinase gene in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 2 ·1981-02-00 ·Pages 815-9

Garapin AC, Colbère-Garapin F, Cohen-Solal M, Horodniceanu F, Kourilsky P

Abstract

A herpes simplex virus type I DNA fragment containing the sequence coding for thymidine kinase was fused to the very beginning of the Escherichia coli lac Z gene in the three possible reading frames. When the thymidine kinase sequence was in the orientation fit to be transcribed from the lac promoter, functional thymidine kinase was made under lac control in all three cases. Sequences data indicate that translation reinitiation occurs at the 5' end of the thymidine kinase gene after stop signals. Two T+A-rich sequences, which may be part of eukaryotic promoters, are found in the same region.

MeSH Terms

Animals Base Sequence Cell Transformation, Viral Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/enzymology L Cells/metabolism Mice Operon Plasmids Protein Biosynthesis Simplexvirus/enzymology Thymidine Kinase/genetics Transcription, Genetic

Chemicals

DNA, Recombinant Thymidine Kinase DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Garapin A C

Colbère-Garapin F

Cohen-Solal M

Horodniceanu F

Kourilsky P

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.2.815

Published

1981-02-00

Pages

815-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC319893

Subset

Databases

GENBANK

V00463

External Links

PubMed source DOI: 10.1073/pnas.78.2.815 PMC Full Text View journal details

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Expression of herpes simplex virus type I thymidine kinase gene in Escherichia coli.

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