Home LiteratureArticle Details
PMID: 6283181 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Synthesis of human adenovirus early RNA species is similar in productive and abortive infections of monkey and human cells.

Journal of virology ·Vol. 42 ·No. 2 ·1982-05-00 ·Pages 748-54

Anderson KP, Klessig DF

Abstract

Northern (RNA) blot analysis has been used to show that synthesis of early mRNA species is similar in monkey cells productively or abortively infected with human adenovirus. mRNA species from all five major early regions (1A, 1B, 2, 3, 4) are identical in size and comparable in abundance whether isolated from monkey cells infected with adenovirus type 2 or with the host range mutant Ad2hr400 or coinfected with adenovirus type 2 plus simian virus 40. The mRNA species isolated from monkey cells are identical in size to those isolated from human cells. Production of virus-associated RNA is also identical in productive and abortive infections of monkey cells. Synthesis of virus-associated RNA is, however, significantly greater in HeLa cells than in CV1 cells at late times after infection regardless of which virus is used in the infection.

MeSH Terms
Adenoviruses, Human/genetics Animals Cell Line Chlorocebus aethiops HeLa Cells/metabolism Helper Viruses/genetics Humans Kidney RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Simian virus 40/genetics Species Specificity Time Factors Virus Replication
Chemicals
RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Anderson K P
Klessig D F
References (37)
37 references, click to expand
  1. Nucleic acid homology studies of adenovirus type 7-SV40 interactions.
    Proc Natl Acad Sci U S A. 1966 Feb;55(2):336-41 PMID: 4287386
  2. A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128-35 PMID: 4311250
  3. Biochemical consequences of type 2 adenovirus and Simian virus 40 double infections of African green monkey kidney cells.
    J Virol. 1970 May;5(5):586-97 PMID: 4315958
  4. The nucleotide sequence of a low molecular weight ribonucleic acid from cells infected with adenovirus 2.
    J Biol Chem. 1971 Nov 25;246(22):6991-7009 PMID: 4331202
  5. Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40.
    J Virol. 1972 Aug;10(2):211-9 PMID: 4342239
  6. Synthesis of viral ribonucleic acid during restricted adenovirus infection.
    J Virol. 1972 Aug;10(2):220-7 PMID: 4342240
  7. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties.
    J Virol. 1973 May;11(5):655-64 PMID: 4350710
  8. Use of nondefective adenovirus-simian virus 40 hybrids for mapping the simian virus 40 genome.
    J Virol. 1973 Sep;12(3):643-52 PMID: 4355861
  9. Complementation of translational defect for growth of human adenovirus type 2 in Simian cells by a Simian virus 40-induced factor.
    J Mol Biol. 1973 Dec 5;81(2):207-23 PMID: 4360026
  10. Conditional lethal mutants of adenovirus 2-simian virus 40 hybrids. I. Host range mutants of Ad2+ND1.
    J Virol. 1974 Jun;13(6):1237-44 PMID: 4364898
  11. Genes for VA-RNA in adenovirus 2.
    Cell. 1975 Oct;6(2):223-9 PMID: 1182803
  12. Block to multiplication of adenovirus serotype 2 in monkey cells.
    J Virol. 1975 Dec;16(6):1650-68 PMID: 172661
  13. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  14. Detection of adenovirus type 2-induced early polypeptides using cycloheximide pretreatment to enhance viral protein synthesis.
    J Virol. 1976 Jul;19(1):232-42 PMID: 950686
  15. Adenovirus transcription. V. Quantitation of viral RNA sequences in adenovirus 2-infected and transformed cells.
    J Mol Biol. 1976 Sep 25;106(3):749-74 PMID: 185395
  16. Isolation of a variant of human adenovirus serotype 2 that multiplies efficiently on monkey cells.
    J Virol. 1977 Mar;21(3):1243-6 PMID: 403299
  17. Adenovirus type 2 early nuclear and mRNA: kinetic estimation of l anf r DNA strand fractions complementary to different abundance classes of viral RNA.
    J Virol. 1977 Sep;23(3):616-25 PMID: 894792
  18. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  19. Loop structures in hybrids of early RNA and the separated strands of adenovirus DNA.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4392-5 PMID: 270682
  20. Possible role of the 72,000 dalton DNA-binding protein in regulation of adenovirus type 5 early gene expression.
    J Virol. 1978 Feb;25(2):664-74 PMID: 203722
  21. The low molecular weight of RNAs of adenovirus 2-infected cells.
    J Mol Biol. 1978 Feb 25;119(2):293-328 PMID: 633371
  22. Structure of the adenovirus 2 early mRNAs.
    Cell. 1978 Jul;14(3):695-711 PMID: 688389
  23. Transcription of adenovirus RNA in permissive and nonpermissive infections.
    J Virol. 1978 Jul;27(1):136-48 PMID: 691108
  24. Autoregulation of adenovirus type 5 early gene expression II. Effect of temperature-sensitive early mutations on virus RNA accumulation.
    J Virol. 1978 Nov;28(2):450-6 PMID: 214573
  25. Regulation of the appearance of cytoplasmic RNAs from region 1 of the adenovirus 2 genome.
    J Mol Biol. 1978 Dec 15;126(3):395-414 PMID: 745234
  26. Autoregulation of adenovirus type 5 early gene expression. III. Transcription studies in isolated nuclei.
    J Virol. 1979 Feb;29(2):458-65 PMID: 430604
  27. Location of additional early gene sequences in the adenoviral chromosome.
    Cell. 1979 Aug;17(4):945-56 PMID: 487438
  28. Mutations that allow human Ad2 and Ad5 to express late genes in monkey cells map in the viral gene encoding the 72K DNA binding protein.
    Cell. 1979 Aug;17(4):957-66 PMID: 114304
  29. Complex splicing patterns of RNAs from the early regions of adenovirus-2.
    J Mol Biol. 1979 Oct 25;134(2):265-303 PMID: 537065
  30. The structure of adenovirus 2 early nuclear and cytoplasmic RNAs.
    J Mol Biol. 1980 Feb 15;137(1):23-48 PMID: 7365795
  31. Regulation of early adenovirus transcription: a protein product of early region 2 specifically represses region 4 transcription.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1893-7 PMID: 6929526
  32. Structure of genes for virus-associated RNAI and RNAII of adenovirus type 2.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2424-8 PMID: 6930642
  33. Binding of adenovirus VA RNA to mRNA: a possible role in splicing?
    Nature. 1980 Jun 19;285(5766):575-7 PMID: 7402301
  34. Identification of the gene and mRNA for the adenovirus terminal protein precursor.
    Cell. 1981 Feb;23(2):497-508 PMID: 7471210
  35. Control of messenger RNA concentration by differential cytoplasmic half-life. Adenovirus messenger RNAs from transcription units 1A and 1B.
    J Mol Biol. 1981 May 25;148(3):231-51 PMID: 7299816
  36. The stability of early adenovirus mRNA is controlled by the viral 72 kd DNA-binding protein.
    Cell. 1981 Nov;26(3 Pt 1):371-9 PMID: 6895718
  37. ENHANCEMENT OF ADENOVIRUS GROWTH IN AFRICAN GREEN MONKEY KIDNEY CELL CULTURES BY SV40.
    Proc Soc Exp Biol Med. 1964 May;116:187-90 PMID: 14200102
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.42.2.748-754.1982
Published
1982-05-00
Pages
748-54
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256902
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.42.2.748-754.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2