ColE1 copy number mutants.
A deletion mutant of the colicin E1-derived plasmid, pDMS6642, exhibited an approximately fourfold increase in copy number. We subsequently isolated hydroxylamine-induced mutants of that plasmid that had a further increase in copy number. Analysis of them suggests that the increased copy number of pDMS6642 is associated with transcriptional readthrough from a Tn3 transposon into the region of ColE1 containing information that influences plasmid replication. The hydroxylamine mutation in one copy number mutant appeared to increase the plasmid copy number by stimulating readthrough transcription from the Tn3 transposon into the ColE1 replication control region, whereas the other hydroxylamine mutation acts by another mechanism.
23 references, click to expand
-
Calcium-dependent bacteriophage DNA infection.J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
-
Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome.J Bacteriol. 1972 May;110(2):562-9 PMID: 4553836
-
Analysis by transformation of the penicillinase system in Bacillus licheniformis.J Gen Microbiol. 1973 May;76(1):217-30 PMID: 4723069
-
Origin of the TEM-beta-lactamase gene found on plasmids.J Bacteriol. 1975 Apr;122(1):250-6 PMID: 1091625
-
Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.Nature. 1976 Jan 29;259(5541):285-90 PMID: 765836
-
Mutations of temperature sensitivity in R plasmid pSC101.J Bacteriol. 1977 Aug;131(2):405-12 PMID: 328479
-
Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion.J Bacteriol. 1977 Oct;132(1):321-31 PMID: 334728
-
Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid.J Bacteriol. 1977 Oct;132(1):332-40 PMID: 334729
-
Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41 PMID: 358200
-
Isolation and characterization of replication-deficient mutants of ColE1 plasmids.J Bacteriol. 1979 Aug;139(2):597-607 PMID: 378979
-
ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.J Bacteriol. 1979 Aug;139(2):608-19 PMID: 378980
-
Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.Mol Gen Genet. 1979 May 4;172(2):151-9 PMID: 384144
-
Plasmid vehicles for direct cloning of Escherichia coli promoters.J Bacteriol. 1979 Nov;140(2):400-7 PMID: 387734
-
Role of plasmid-coded RNA and ribonuclease III in plasmid DNA replication.Cell. 1979 Sep;18(1):61-71 PMID: 389434
-
Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9.Nature. 1979 Dec 20-27;282(5741):864-9 PMID: 390403
-
DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3.Cell. 1979 Dec;18(4):1153-63 PMID: 391406
-
Nucleotide sequence of the region required for maintenance of colicin E1 plasmid.Mol Gen Genet. 1979 Oct 3;176(2):161-70 PMID: 393952
-
Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
-
Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
-
Functional relationship between parts of the replication region of plasmid ColE1.J Bacteriol. 1980 Aug;143(2):1073-6 PMID: 7009549
-
Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA.Proc Natl Acad Sci U S A. 1981 Mar;78(3):1421-5 PMID: 6165011
-
A single base-pair alteration is responsible for the DNA overproduction phenotype of a plasmid copy-number mutant.Cell. 1981 Apr;24(1):235-42 PMID: 6263488
-
Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication.J Bacteriol. 1981 Sep;147(3):962-71 PMID: 6268615
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
