The DNA tumor virus SV 40 induces gene mutations in human cells. Reversion of HPRT deficiency.

Human genetics ·Vol. 61 ·No. 3 ·1982-00-00 ·Pages 236-41

Lübbe L, Strauss M, Scherneck S, Geissler E

Abstract

The mutagenic effect of papovavirus SV40 on human cells could be demonstrated by reversion of HPRT deficiency in Lesch-Nyhan fibroblasts transformed by the virus. SV40 seems to induce different gene mutations in individually selected cell clones, as was clearly shown by the respective HPRT enzyme properties. The consequences of our results for use of SV40-derived vectors in gene substitution experiments are discussed.

MeSH Terms

Cell Transformation, Viral Genetic Vectors Humans Hypoxanthine Phosphoribosyltransferase/deficiency,genetics Lesch-Nyhan Syndrome/enzymology Mutation Simian virus 40/genetics

Chemicals

Hypoxanthine Phosphoribosyltransferase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lübbe L

Strauss M

Scherneck S

Geissler E

References (31)

31 references, click to expand

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Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00296449

Published

1982-00-00

Pages

236-41

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00296449 View journal details

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The DNA tumor virus SV 40 induces gene mutations in human cells. Reversion of HPRT deficiency.

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