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PMID: 6303211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tn2011, a new transposon encoding oxacillin-hydrolyzing beta-lactamase.

Antimicrobial agents and chemotherapy ·Vol. 23 ·No. 3 ·1983-03-00 ·Pages 407-12

Nakazawa H, Mitsuhashi S

Abstract

The type II penicillinase (oxacillin-hydrolyzing beta-lactamase, OXA-1) gene on plasmid Rms213 was transposed to various plasmids or to the host chromosome. The transposon bearing this gene, designated Tn2011, conferred resistance to ampicillin, streptomycin, sulfonamide, and mercuric chloride. By restriction endonuclease digestion and agarose gel electrophoresis, the molecular weight of Tn2011 was estimated to be 12.5 X 10(6). The transposition frequency of Tn2011 was about 10(-4) to 10(-5). The activity of type II penicillinase is related to the copy number of the replicon bearing Tn2011.

MeSH Terms
Ampicillin/pharmacology DNA Transposable Elements Hydrolysis Microscopy, Electron Oxacillin/metabolism R Factors beta-Lactamases/biosynthesis,genetics
Chemicals
DNA Transposable Elements Ampicillin beta-Lactamases Oxacillin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nakazawa H
Mitsuhashi S
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21 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.23.3.407
Published
1983-03-00
Pages
407-12
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC184661
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.23.3.407 PMC Full Text View journal details
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