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PMID: 6306397 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The use of transposon insertion zdc-235::Tn10 (min 32) to clone and delete DNA from the terminus region of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 189 ·No. 3 ·1983-00-00 ·Pages 506-12

Henson JM, Kuempel PL

Abstract

Transposon zdc-235::Tn10 is inserted at min 32 on the genetic map of Escherichia coli, and we have used this transposon to clone 14 kb of DNA that flanks this insertion. The site of insertion of the transposon, and the restriction map of the cloned DNA, correspond well with the predictions of the Bouché restriction map for the terminus region (Bouché 1982). The zdc-235::Tn10 insertion, along with the zdd-230::Tn9 insertion, was used to obtain deletions of the region that has been cloned. Strains lacking a minimum of 14 kb, and more likely a minimum of 40 kb of DNA, showed no alteration of growth or cell morphology.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial/ultrastructure Cloning, Molecular/methods DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/genetics,ultrastructure Genetic Vectors Nucleic Acid Hybridization
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Henson J M
Kuempel P L
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00325918
Published
1983-00-00
Pages
506-12
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00325918
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