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PMID: 6308396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 190 ·No. 3 ·1983-00-00 ·Pages 403-5

Buxton FP, Radford A

Abstract

A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'-monophosphate carboxylase in E. coli is within the Neurospora sequence.

MeSH Terms
Carboxy-Lyases/genetics Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes Genetic Complementation Test Neurospora/genetics Neurospora crassa/genetics Nucleic Acid Hybridization Orotidine-5'-Phosphate Decarboxylase/genetics Transformation, Genetic
Chemicals
DNA Restriction Enzymes Carboxy-Lyases Orotidine-5'-Phosphate Decarboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Buxton F P
Radford A
References (11)
11 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331067
Published
1983-00-00
Pages
403-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331067
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