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PMID: 6328431 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of the fumarase gene fumA, of Escherichia coli.

Nucleic acids research ·Vol. 12 ·No. 8 ·1984-04-25 ·Pages 3631-42

Miles JS, Guest JR

Abstract

The nucleotide sequence of a 2.41 kb fragment of E. coli DNA containing the fumA gene encoding fumarase was determined using the dideoxy chain termination method. The initiation and termination sites of fumA transcription were located using RNA:DNA hybridisation with single-stranded M13 probes. The length of the fumA transcript was estimated as 1760 +/- 15 nucleotides and the fumA coding region of 1647 base pairs (549 codons) was identified. The deduced molecular weight of the fumarase protein (Mr = 60163) is in good agreement with that of the protein identified by the maxicell procedure. The DNA fragment also contains the 5' end of the manA gene encoding mannose 6-phosphate isomerase, which is transcribed with the opposite polarity to fumA .

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes Escherichia coli/enzymology,genetics Fumarate Hydratase/genetics Genes Genes, Bacterial Nucleic Acid Hybridization Transcription, Genetic
Chemicals
DNA Restriction Enzymes Fumarate Hydratase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miles J S
Guest J R
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.8.3631
Published
1984-04-25
Pages
3631-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318774
Subset
IM
Databases
GENBANK
X00522
External Links
PubMed source DOI: 10.1093/nar/12.8.3631 PMC Full Text View journal details
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