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PMID: 6328436 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A multigene family of intron lacking and containing genes, encoding for mouse ribosomal protein L7.

Nucleic acids research ·Vol. 12 ·No. 9 ·1984-05-11 ·Pages 3763-76

Klein A, Meyuhas O

Abstract

Mouse ribosomal protein L7 is encoded by a multigene family. Screening of two mouse genomic libraries with cloned L7 cDNA, has resulted in the isolation of nine independent lambda Charon 4A recombinant phages which include seven different L7 genes. Restriction enzyme mapping of six of these genes (L7-1, L7-16, L7-18, L7-28, L7-35 and L7- 16b ) reveals dissimilarity in sites within the L7 sequences as well as in the flanking regions. Electron microscopic analysis of heteroduplex and S1 nuclease mapping demonstrate that the first five genes contain the entire L7 mRNA sequence but lack introns. Based on these features we propose that these are processed genes. Of the L7 genes described here only one (L7- 16b ) exhibits a high degree of homology with L7 mRNA and contains introns. We discuss the possibility that this low representation of intron containing L7 genes may reflect the proportion of functional L7 genes in this multigene family.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes Liver/metabolism Male Mice Mice, Inbred BALB C Mice, Inbred Strains Microscopy, Electron Plasmids RNA, Messenger/genetics Ribosomal Proteins/genetics
Chemicals
RNA, Messenger Ribosomal Proteins ribosomal protein L7-L12 DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klein A
Meyuhas O
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.9.3763
Published
1984-05-11
Pages
3763-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318788
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.9.3763 PMC Full Text View journal details
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