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PMID: 6328445 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance.

Nucleic acids research ·Vol. 12 ·No. 9 ·1984-05-11 ·Pages 3917-32

Sharpe CR, Sidoli A, Shelley CS, Lucero MA, Shoulders CC, Baralle FE

Abstract

The structure and function of the genes encoding the polypeptide components of plasma lipoproteins are of interest because of the central role they play in the regulation of lipid metabolism. We have now completed our previous studies on the human apoAI gene and furthermore isolated and sequenced cDNA clones for apoAII , CII and CIII. The nucleotide sequences show the signal peptides of apoAII , CII and CIII to be 18, 22 and 20 amino acids in length, respectively, and in addition that prepro apoAII bears a classical propeptide structure of 5 amino acids. The amino acid homology detected between apoCII and pro- apoAI is discussed, as is the gene arrangement of the 5' non-coding region of apoAI mRNA. The relative liver mRNA levels of the 4 apolipoproteins analysed in this study have been estimated and compared with their corresponding plasma products. The data reported here provide an essential basis for further studies of structural and functional alleles of apo AI, AII, CII and CIII genes.

MeSH Terms
Amino Acid Sequence Apolipoprotein A-I Apolipoprotein A-II Apolipoprotein C-II Apolipoprotein C-III Apolipoproteins/genetics Apolipoproteins C Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Genes Humans Liver/metabolism Oligodeoxyribonucleotides/analysis RNA, Messenger/genetics
Chemicals
Apolipoprotein A-I Apolipoprotein A-II Apolipoprotein C-II Apolipoprotein C-III Apolipoproteins Apolipoproteins C Oligodeoxyribonucleotides RNA, Messenger DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sharpe C R
Sidoli A
Shelley C S
Lucero M A
Shoulders C C
Baralle F E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.9.3917
Published
1984-05-11
Pages
3917-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318799
Subset
IM
Databases
GENBANK
J00098, J00099, J00100, J00101, J03222, K00946, K01518, K01686, K02045, K02092, K02216, M10372, M10612, X00566, X00567, X00568, X00569, X00927
External Links
PubMed source DOI: 10.1093/nar/12.9.3917 PMC Full Text View journal details
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