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PMID: 6340066 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nuclease mapping of the secondary structure of the 49-nucleotide 3' terminal cloacin fragment of Escherichia coli 16s RNA and its interactions with initiation factor 3.

Nucleic acids research ·Vol. 11 ·No. 7 ·1983-04-11 ·Pages 2035-52

Wickstrom E

Abstract

Escherichia coli translational initiation factor 3 (IF3) may be crosslinked to the 3' end of 16S RNA in 30S ribosomal subunits. In order to determine the sequence to which IF3 may bind in vivo, samples of 5'-32P labelled 3' terminal 49-nucleotide fragment of 16S RNA were incubated 5 min. at 37 degrees in 40 mM Tris-HOAc, pH 7.4, 100 mM NaCl, 1 mM Mg (OAc)2, 1 mM ZnSO4, with or without IF3, then reacted a further 5 min with nuclease S1, RNase T1, or RNase A. Base pairing between the 5' and 3' legs of the fragment occurs in the absence of IF3, but is disrupted by IF3 binding. IF3 appears to protect some residues near the 5' end of the fragment (U1495, A1499, A1500, A1502, and A1503) from nuclease S1, and potentiates S1 attack on others (G1494, G1497, C1501, G1504, G1505, U1506, G1517, G1529, G1530, and C1533). A series of equimolar reactions at increasing dilution imply an association constant range of 1.4-7.0 X 10(7) M-1.

MeSH Terms
Bacteriocins/genetics Base Sequence Cloacin/genetics Escherichia coli/genetics Nucleic Acid Conformation Peptide Initiation Factors/genetics Prokaryotic Initiation Factor-3 RNA, Ribosomal/genetics Ribosomal Proteins/genetics
Chemicals
Bacteriocins Peptide Initiation Factors Prokaryotic Initiation Factor-3 RNA, Ribosomal Ribosomal Proteins Cloacin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wickstrom E
References (44)
44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.7.2035
Published
1983-04-11
Pages
2035-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325860
Subset
IM
Grants
NIGMS NIH HHS · GM-24128 · United States
NIGMS NIH HHS · GM-28408 · United States
External Links
PubMed source DOI: 10.1093/nar/11.7.2035 PMC Full Text View journal details
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