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PMID: 6361061 Published · ppublish English Comparative Study Journal Article

Comparative evaluation of different enzyme-linked immunosorbent assay systems for the detection of staphylococcal enterotoxins A, B, C, and D.

Journal of clinical microbiology ·Vol. 19 ·No. 1 ·1984-01-00 ·Pages 34-8

Fey H, Pfister H, Rüegg O

Abstract

We compared four versions of the enzyme-linked immunosorbent assay for their suitability for detecting staphylococcal enterotoxins. The sandwich with labeled antibody proved to be the best. We used it with a sorbent consisting of antibody-coated polystyrene spheres reacted with 20 ml of food extract. The sensitivity of the test was 0.1 ng of enterotoxin per ml, which is far below clinical relevance. The succinimidyl-pyridyl-dithio-propionate enzyme coupling method of Pharmacia was superior to the two-step glutaraldehyde technique. Interfering protein A was eliminated by the simple addition of normal rabbit serum to the extracts. A diagnostic kit is now available.

MeSH Terms
Enterotoxins/analysis Enzyme-Linked Immunosorbent Assay Evaluation Studies as Topic Immunoenzyme Techniques Staphylococcus aureus
Chemicals
Enterotoxins enterotoxin D, Staphylococcal enterotoxin A, Staphylococcal enterotoxin B, staphylococcal enterotoxin C, staphylococcal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fey H
Pfister H
Rüegg O
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26 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.19.1.34-38.1984
Published
1984-01-00
Pages
34-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC270973
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.19.1.34-38.1984 PMC Full Text View journal details
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