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PMID: 6374375 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multiple effects of kanamycin on translational accuracy.

Molecular & general genetics : MGG ·Vol. 194 ·No. 1-2 ·1984-00-00 ·Pages 195-9

Jelenc PC, Kurland CG

Abstract

We have studied the effects of kanamycin on the accuracy of translation in vitro by wild-type and mutant ribosomes from Escherichia coli. Kanamycin stimulates the leucine missense error of poly(U) translation by wild-type, Ram, and streptomycin-resistant ribosomes in characteristic ways; in particular, the streptomycin-resistant ribosomes are significantly less error-prone than wild-type or Ram ribosomes at all concentrations of the antibiotic. Kinetic analysis of the effects of kanamycin on the translational accuracy of wild-type ribosomes reveals a different concentration dependence for the perturbation of the initial selectivity of streptomycin-resistant ribosomes is not affected by kanamycin; the drug enhances only the error of proofreading by this mutant ribosome. We suggest that the multiple effects of kanamycin on the errors of translation are due to separate effects at different ribosomal sites.

MeSH Terms
Bacterial Proteins/biosynthesis Codon Escherichia coli/drug effects,genetics Kanamycin/pharmacology Leucine/metabolism Protein Biosynthesis/drug effects Ribosomes/drug effects Streptomycin/pharmacology
Chemicals
Bacterial Proteins Codon Kanamycin Leucine Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jelenc P C
Kurland C G
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00383516
Published
1984-00-00
Pages
195-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00383516
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