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PMID: 6387394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Undermodification in the first position of the anticodon of supG-tRNA reduces translational efficiency.

Molecular & general genetics : MGG ·Vol. 196 ·No. 2 ·1984-00-00 ·Pages 194-200

Hagervall TG, Björk GR

Abstract

Two mutants of Escherichia coli, trmC1 and trmC2, which are both defective in the synthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) were utilized to study the function of this complex modified nucleoside. Transfer RNAs specific for glutamine, glutamic acid and lysine as well as a specific ochre suppressor derived from lysine tRNA (tRNAUAAlys encoded by the supG allele), contain this modified nucleoside at position 34 (the wobble position). It was found that two different undermodified derivatives of mnm5s2U were present in the two trmC mutants, which suggests that the two mutations affect two different enzymatic activities. Using the lacI-Z fusion system (Miller and Albertini 1983), we found that the efficiency of supG-mediated suppression was reduced to 30%-90% of the wild-type value in the trmC mutants. The modification-deficient supG-tRNA in the mutants showed a higher sensitivity to codon context than the normal tRNAUAAlys.

MeSH Terms
Anticodon Bacterial Proteins/genetics Escherichia coli/genetics Genes, Bacterial Genetic Complementation Test Methylation Plasmids Protein Biosynthesis RNA, Transfer/genetics Suppression, Genetic Thiouridine/analogs & derivatives,biosynthesis tRNA Methyltransferases/genetics
Chemicals
Anticodon Bacterial Proteins Thiouridine 5-methylaminomethyl-2-thiouridine RNA, Transfer tRNA Methyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hagervall T G
Björk G R
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328050
Published
1984-00-00
Pages
194-200
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00328050
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