Relaxation of supercoiled plasmid DNA by oxidative stresses in Escherichia coli.

Journal of bacteriology ·Vol. 160 ·No. 3 ·1984-12-00 ·Pages 1017-21

Horiuchi H, Takagi M, Yano K

Abstract

The relaxation of plasmid DNA was observed after the visible light irradiation of Escherichia coli AB1157 harboring plasmid pBR322 or some other plasmids in the presence of a photosensitizing dye, such as toluidine blue or acridine orange, and molecular oxygen. Treatment of the cells with hydroperoxides, such as tert-butyl hydroperoxide, cumene hydroperoxide, and hydrogen peroxide, also caused the plasmid DNA relaxation in vivo. Relaxation was not observed in these treatments of purified pBR322 DNA in vitro. Plasmid DNA relaxation was also detected after near-UV irradiation. Far-UV irradiation did not induce such relaxation.

MeSH Terms

DNA, Superhelical/metabolism,radiation effects Escherichia coli/genetics,metabolism,radiation effects Oxidation-Reduction Peroxides/pharmacology Photochemistry Plasmids/radiation effects Ultraviolet Rays

Chemicals

DNA, Superhelical Peroxides

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Horiuchi H

Takagi M

Yano K

References (28)

28 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.160.3.1017-1021.1984

Published

1984-12-00

Pages

1017-21

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215811

Subset

External Links

PubMed source DOI: 10.1128/jb.160.3.1017-1021.1984 PMC Full Text View journal details

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Relaxation of supercoiled plasmid DNA by oxidative stresses in Escherichia coli.

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