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PMID: 6425831 Published · ppublish English Comparative Study Journal Article

Immunological evidence for the in vivo occurrence of (2'-5')adenylyladenosine oligonucleotides in eukaryotes and prokaryotes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 8 ·1984-04-00 ·Pages 2322-6

Laurence L, Marti J, Roux D, Cailla H

Abstract

A monoclonal antibody highly specific for (2'-5')adenylyladenosine oligonucleotides was used together with a 125I-labeled analog of this compound to detect and quantify phosphorylated and nonphosphorylated (2'-5')adenylyladenosine oligonucleotides in a variety of tissues and cells. These oligonucleotides were first assayed as a whole in perchloric acid extracts and then further individually characterized by HPLC analysis. Their sensitivity to alkaline phosphatase, snake venom phosphodiesterase, and T2 RNase was systematically checked. Nonphosphorylated (2'-5')adenylyladenosine oligonucleotides were found in mammalian tissues as well as in yeast and bacteria. In normal mouse brain, lung, heart, pancreas, spleen, kidney, and liver their concentrations ranged from 10 to 200 pmol/g wet weight, depending on tissue and strain. The oligonucleotides were mainly dimers, trimers, tetramers, and pentamers. In addition, phosphorylated (2'-5')adenylyladenosine oligonucleotides were shown in liver and kidney extracts.

MeSH Terms
Adenine Nucleotides/analysis,immunology Animals Antibodies, Monoclonal Antigen-Antibody Complex Cell Line Chromatography, High Pressure Liquid Cross Reactions Drosophila melanogaster/analysis Escherichia coli/analysis Fungi/analysis Humans Mice Mice, Inbred C3H Oligonucleotides/analysis Oligoribonucleotides/analysis,immunology Radioimmunoassay Rats Rats, Inbred Strains Species Specificity Tissue Distribution
Chemicals
Adenine Nucleotides Antibodies, Monoclonal Antigen-Antibody Complex Oligonucleotides Oligoribonucleotides 2',5'-oligoadenylate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Laurence L
Marti J
Roux D
Cailla H
References (35)
35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.8.2322
Published
1984-04-00
Pages
2322-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345051
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.8.2322 PMC Full Text View journal details
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