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PMID: 6480109 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell surface proteins of oral streptococci.

Infection and immunity ·Vol. 46 ·No. 1 ·1984-10-00 ·Pages 245-50

Appelbaum B, Rosan B

Abstract

Whole cells of representative strains of oral streptococci (Streptococcus sanguis, S. mitis, and S. salivarius) were radiolabeled by the lactoperoxidase method of radioiodination. The labeled polypeptides obtained by extraction of whole cells with boiling sodium dodecyl sulfate were analyzed by polyacrylamide gel electrophoresis and autoradiography. Of the total radioactivity, ca. 70% was released by treating whole cells with trypsin, suggesting that the labeling was confined to proteins located on the cell surface. Most S. sanguis strains studied gave a characteristic banding pattern consisting of a high-molecular-weight (120,000 [120K] to 63K) group of six proteins. Three low-molecular-weight (12K, 16K, and 18K) proteins were also detected in many strains.

MeSH Terms
Bacterial Proteins/analysis Molecular Weight Mouth/microbiology Streptococcus/analysis,classification Streptococcus mutans/analysis Streptococcus sanguis/analysis Trypsin
Chemicals
Bacterial Proteins Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Appelbaum B
Rosan B
References (41)
41 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.46.1.245-250.1984
Published
1984-10-00
Pages
245-50
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261463
Subset
IM
Grants
NIDCR NIH HHS · DE-02623 · United States
NIDCR NIH HHS · DE-03180 · United States
NIDCR NIH HHS · DE-06395 · United States
External Links
PubMed source DOI: 10.1128/iai.46.1.245-250.1984 PMC Full Text View journal details
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