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PMID: 6766853 Published · ppublish English Journal Article

Hybridization of tRNAs of Drosophila melanogaster to polytene chromosomes.

Chromosoma ·Vol. 76 ·No. 1 ·1980-00-00 ·Pages 65-84

Hayashi S, Gillam IC, Delaney AD, Dunn R, Tener GM, Grigliatti TA, Suzuki DT

Abstract

Highly purified tRNAs from Drosophila melanogaster were iodinated with 125I and hybridized to squashes of polytene chromosomes of Drosophila silivary glands followed by autoradiography to localize binding sites. Most tRNAs hybridize strongly to more than one site and weakly to one or more additional sites. The major sites for various tRNAs are the following: tRNA2Arg, 42A, 84F1,2; tRNA2Asp, 29DE; tRNA3Gly, 22BC, 35BC, 57BC, tRNA2Lys, 42A, 42E; tRNA5Lys, 84AB, 87B; tRNA2Met, 48B5-7, 72F1-2, 83F-84A; tRNA3Met, 46A1-2, 61D1-2, 70F1-2; tRNA4Ser, 12DE, 23E; tRNA7Ser, 12DE, 23E; tRNA3aVal, 64D; tRNA3bVal, 84d3-4, 92b1-9; tRNA4Val, 56D3-7, 70BC.

MeSH Terms
Animals Autoradiography Chromosomes/metabolism DNA/metabolism Drosophila melanogaster/genetics Nucleic Acid Hybridization RNA, Transfer/metabolism
Chemicals
DNA RNA, Transfer
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hayashi S
Gillam I C
Delaney A D
Dunn R
Tener G M
Grigliatti T A
Suzuki D T
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28 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00292227
Published
1980-00-00
Pages
65-84
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292227 View journal details
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