Abstract
Three recombinant plasmids containing randomly sheared genomic D. melanogaster tRNAs have been identified and characterized in detail. One of these, the plasmid 14C4, has a D. melanogaster (Dm) DNA segment of 18 kb, and has three tRNA2Arg and two tRNAAsN genes. The second plasmid, 38B10, has tRNAHis genes, while the third plasmid, 63H5, contains coding sequences for tRNA2Asp. The Dm DNA segments in each recombinant plasmid are derived from unique cytogenetic loci. 14C4 is from 84 F, 38B10 is from 48 F and 63H5 is from 70 A.
MeSH Terms
Animals
Arginine
Asparagine
Aspartic Acid
Base Sequence
DNA, Recombinant/metabolism
Drosophila melanogaster/metabolism
Escherichia coli/metabolism
Genes
Microscopy, Electron
Molecular Weight
Nucleic Acid Conformation
Nucleic Acid Hybridization
Plasmids
RNA, Transfer/biosynthesis
Chemicals
DNA, Recombinant
Aspartic Acid
Asparagine
RNA, Transfer
Arginine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Dudler R
Egg A H
Kubli E
Artavanis-Tsakonas S
Gehring W J
Steward R
Schedl P
References (34)
34 references, click to expand
-
Separation of transfer ribonucleic acids by reverse phase chromatography.
J Biol Chem. 1965 Oct;240(10):3979-83
PMID: 5320645
-
On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA.
Natl Cancer Inst Monogr. 1966 Dec;23:449-72
PMID: 5963988
-
The organization of genetic units in chromosomes.
J Cell Sci. 1967 Mar;2(1):1-7
PMID: 6031004
-
Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels.
J Mol Biol. 1969 Apr 14;41(1):139-47
PMID: 4979520
-
Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids.
Anal Biochem. 1971 Dec;44(2):486-95
PMID: 4943341
-
Localization of tRNA genes in the salivary chromosomes of Drosophila by RNA:DNA hybridization.
Genetics. 1971 Oct;69(2):163-78
PMID: 5002748
-
Chromatography of Drosophila tRNA on BD-cellulose.
Can J Biochem. 1973 Jun;51(6):896-902
PMID: 4197825
-
Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus.
J Mol Biol. 1973 Mar 15;74(4):635-51
PMID: 4199662
-
Enzymatic end-to end joining of DNA molecules.
J Mol Biol. 1973 Aug 15;78(3):453-71
PMID: 4754844
-
Reiterated transfer RNA genes of Xenopus laevis.
J Mol Biol. 1973 Sep 15;79(2):391-410
PMID: 4760135
-
Studies on the transfer RNA genes of Drosophila.
Cold Spring Harb Symp Quant Biol. 1974;38:461-74
PMID: 4208790
-
Unequal crossover and the evolution of multigene families.
Cold Spring Harb Symp Quant Biol. 1974;38:507-13
PMID: 4524771
-
The localization of transfer RNA5Lys genes in Drosophila melanogaster.
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3527-31
PMID: 4215081
-
Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels.
FEBS Lett. 1975 Feb 1;50(2):185-9
PMID: 1089563
-
An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose.
Nucleic Acids Res. 1975 Jun;2(6):853-64
PMID: 1096084
-
Fractionation of nucleic acids with special regard to rapidly labeled RNA.
Anal Biochem. 1975 Jul;67(1):147-56
PMID: 1147225
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17
PMID: 1195397
-
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5
PMID: 1105573
-
Redundant genes.
Annu Rev Genet. 1975;9:355-85
PMID: 1108767
-
Transcription and processing of transfer RNA precursors.
Prog Nucleic Acid Res Mol Biol. 1976;16:25-73
PMID: 766079
-
The radioiodination of RNA and DNA to high specific activities.
Methods Cell Biol. 1976;13:121-52
PMID: 1263850
-
Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences.
Cell. 1976 Aug;8(4):485-93
PMID: 986255
-
Base sequence complexity of the stable RNA species of Drosophila melanogaster.
Biochemistry. 1976 Dec 14;15(25):5511-9
PMID: 826268
-
The structural organization of ribosomal DNA in Drosophila melanogaster.
Cell. 1977 Feb;10(2):193-212
PMID: 402222
-
The sequence arrangement of Drosophila melanogaster 5s DNA cloned in recombinant plasmids.
Cell. 1977 Jul;11(3):585-98
PMID: 407004
-
Sequence arrangement of tRNA genes on a fragment of Drosophila melanogaster DNA cloned in E. coli.
Cell. 1977 Aug;11(4):763-77
PMID: 408014
-
The 5S genes of Drosophila melanogaster.
Cell. 1977 Dec;12(4):1057-67
PMID: 413625
-
The localization of tRNA4Glu genes from Drosophila melanogaster by "in situ" hybridization.
Nucleic Acids Res. 1978 May;5(5):1465-78
PMID: 96430
-
The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization.
Mol Gen Genet. 1978 Sep 8;164(3):249-54
PMID: 101767
-
On spacers.
Cell. 1979 Apr;16(4):697-710
PMID: 378397
-
Histone genes and histone messengers.
Annu Rev Biochem. 1979;48:837-70
PMID: 112914
-
Isolation and characterization of recombinant DNA plasmids carrying Drosophila tRNA genes.
Gene. 1979 Nov;7(3-4):197-215
PMID: 118082
-
Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.
Cell. 1980 Feb;19(2):345-53
PMID: 7357612
-
A new method for the isolation of ribonucleic acids from mammalian tissues.
Biochem J. 1956 Nov;64(3):405-8
PMID: 13373784