Home LiteratureArticle Details
PMID: 6795625 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Initiation factor-independent translation of mRNAs from Gram-positive bacteria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 4912-6

McLaughlin JR, Murray CL, Rabinowitz JC

Abstract

Initiation factor-independent translation of mRNA derived from bacillus phage phi29 DNA occurs with translation systems derived from Bacillus subtilis or Escherichia coli. This is in sharp contrast to the strict dependence on ribosome salt wash fraction of E. coli ribosomes for the translation of T7 and other mRNAs derived from Gram-negative organisms.

MeSH Terms
Bacillus subtilis/genetics Bacteriophages/genetics Escherichia coli/genetics Peptide Initiation Factors/physiology Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Ribosomal Proteins/metabolism Ribosomes/physiology
Chemicals
Peptide Initiation Factors RNA, Messenger RNA, Viral Ribosomal Proteins ribosomal protein S1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McLaughlin J R
Murray C L
Rabinowitz J C
References (42)
42 references, click to expand
  1. Role of ribosomal protein S1 in portein synthesis: effects of its addition to Bacillus stearothermophilus cell-free system.
    Eur J Biochem. 1975 Aug 1;56(1):15-22 PMID: 1100388
  2. Function of Escherichia coli ribosomal protein S1 in translation of natural and synthetic messenger RNA.
    J Mol Biol. 1975 Apr 15;93(3):351-66 PMID: 1095761
  3. The role of IF-3 in the translation of T7- and phi80trp messenger RNA.
    Mol Gen Genet. 1975 Sep 8;139(4):311-9 PMID: 1102944
  4. Specificity of bacterial ribosomes and messenger ribonucleic acids in protein synthesis reactions in vitro.
    J Biol Chem. 1976 Apr 25;251(8):2499-510 PMID: 816792
  5. Selective messenger translation by Bacillus subtilis ribosomes.
    Mol Gen Genet. 1976 Dec 31;142(4):277-87 PMID: 818501
  6. Bacillus subtilis phage phi29. Characterization of gene products and functions.
    Eur J Biochem. 1976 JUL 1;66(2):229-41 PMID: 820555
  7. Purification and properties of two initiation factors from Bacillus stearothermophilus.
    Biochimie. 1976;58(1-2):183-99 PMID: 782553
  8. Initial rate kinetic analysis of the mechanism of initiation complex formation and the role of initiation factor IF-3.
    Biochemistry. 1977 Apr 19;16(8):1684-9 PMID: 322704
  9. DNA replication of bacteriophage phi29. Effect of two viral genes on the association of phage chromosomes with the host cell membrane.
    Virology. 1977 May 1;78(1):234-40 PMID: 404758
  10. Differential requirements for polypeptide chain initiation complex formation at the three bacteriophage R17 initiator regions.
    Nucleic Acids Res. 1977 Jan;4(1):1-15 PMID: 325516
  11. Purification and characterization of protein synthesis initiation factors IF1, IF2, and IF3 from Escherichia coli.
    Arch Biochem Biophys. 1977 Aug;182(2):626-38 PMID: 332085
  12. DNA cloning in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433-6 PMID: 418413
  13. Recombinant plasmids capable to replication in B. subtilis and E. coli.
    Mol Gen Genet. 1978 Jun 1;162(1):59-67 PMID: 97514
  14. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):381-5 PMID: 284354
  15. RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1979 Feb 5;127(4):411-36 PMID: 107317
  16. Purification of Bacillus subtilis RNA polymerase with heparin-agarose. In vitro transcription of phi 29 DNA.
    J Biol Chem. 1979 Sep 25;254(18):9220-6 PMID: 113409
  17. DNA-directed cell-free protein-synthesizing system of Bacillus subtilis.
    Biochim Biophys Acta. 1979 Aug 29;564(1):162-71 PMID: 93969
  18. Protein synthesis in Bacillus subtilis. I. Hydrodynamics and in vitro functional properties of ribosomes from B. subtilis W168.
    J Mol Biol. 1979 Dec 15;135(3):611-26 PMID: 119873
  19. Protein synthesis in Bacillus subtilis. II. Selective translation of natural mRNAs and its possible relation to the species-specific inhibition of protein synthesis by lincomycin and erythromycin.
    J Mol Biol. 1979 Dec 15;135(3):627-38 PMID: 94104
  20. Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin.
    Cell. 1980 Jun;20(2):543-53 PMID: 6248249
  21. The phylogeny of prokaryotes.
    Science. 1980 Jul 25;209(4455):457-63 PMID: 6771870
  22. Specificity of promoter site utilization in vitro by bacterial RNA polymerases on Bacillus phage phi 29 DNA. Transcription mapping with exonuclease III.
    J Biol Chem. 1980 Sep 25;255(18):8819-30 PMID: 6251067
  23. Properties of simian virus 40 small t antigen overproduced in bacteria.
    J Virol. 1981 Feb;37(2):683-97 PMID: 6261013
  24. Specificity in bacterial protein synthesis: role of initiation factors and ribosomal subunits.
    Nature. 1970 May 23;226(5247):705-7 PMID: 4910148
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  26. Evidence against the folate-mediated formylation of formyl-accepting methionyl transfer ribonucleic acid in Streptococcus faecalis R.
    J Biol Chem. 1970 Oct 10;245(19):5115-21 PMID: 4990168
  27. Polyuridylic acid binding and translating by Escherichia coli ribosomes: stimulation by protein I, inhibition by aurintricarboxylic acid.
    Biochim Biophys Acta. 1972 Oct 27;281(3):381-92 PMID: 4565240
  28. Initiation of protein synthesis in vitro by a clostridial system. I. Specificity in the translation of natural messenger ribonucleic acids.
    J Biol Chem. 1973 May 10;248(9):3209-15 PMID: 4573349
  29. Initiation of protein synthesis in vitro by a clostridial system. II. The roles of initiation factors and salt-washed ribosomes in determining specificity in the translation of natural messenger ribonucleic acids.
    J Biol Chem. 1973 May 10;248(9):3216-9 PMID: 4573350
  30. Structural dynamics of bacterial ribosomes. I. Characterization of vacant couples and their relation to complexed ribosomes.
    J Mol Biol. 1973 Apr 5;75(2):281-94 PMID: 4580677
  31. Proteins induced in Bacillus subtilis infected with bacteriophage phi 29.
    Virology. 1973 Nov;56(1):291-9 PMID: 4200881
  32. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  33. Initiation factor-dependent binding of MS2 RNA to 30-S ribosomes and the recycling of IF-3.
    Eur J Biochem. 1973 Dec 3;40(1):295-308 PMID: 4589551
  34. Polypeptide chain initiation in Caulobacter crescentus without initiation factor IF-1.
    J Biol Chem. 1974 Mar 10;249(5):1465-8 PMID: 4594128
  35. Gene expression during the development of bacteriophage phi 29. 3. Analysis of viral-specific protein synthesis with suppressible mutants.
    J Virol. 1974 Mar;13(3):690-8 PMID: 4362871
  36. Role of 16S ribosomal ribonucleic acid and the 30S ribosomal protein S12 in the initiation of natural messenger ribonucleic acid translation.
    Biochemistry. 1974 May 7;13(10):2115-22 PMID: 4597072
  37. Cistron specificity of 30S ribosomes heterologously reconstituted with components from Escherichia coli and Bacillus stearothermophilus.
    Biochemistry. 1974 May 7;13(10):2123-9 PMID: 4597073
  38. Ribsome and messenger specificity in protein synthesis by bacteria.
    Biochem Biophys Res Commun. 1974 May 7;58(1):92-8 PMID: 4208644
  39. Studies on the in vitro synthesis of beta-galactosidase: necessary components in the ribosomal wash.
    Arch Biochem Biophys. 1974 Jun;162(2):578-84 PMID: 4600957
  40. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  41. Interaction of Escherichia coli 30S ribosomal subunits with MS2 phage RNA in the absence of initiation factors.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3611-5 PMID: 4610580
  42. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.8.4912
Published
1981-08-00
Pages
4912-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC320292
Subset
IM
Grants
NIADDK NIH HHS · AM2109 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.8.4912 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2