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PMID: 6897113 Published · ppublish English Journal Article

The location of the first AUG codons in cowpea mosaic virus RNAs.

Nucleic acids research ·Vol. 10 ·No. 16 ·1982-08-25 ·Pages 4861-72

Lomonossoff GP, Shanks M, Matthes HD, Singh M, Gait MJ

Abstract

We have made use of the known sequence of the 5' ends of both CPMV RNAs to synthesise an oligodeoxynucleotide which can prime second-strand DNA synthesis on full-length cDNA copies of both RNAs. By priming synthesis in the presence of dideoxynucleoside triphosphates, we have determined the positions of the first AUG codons in each RNA. These occur at positions 115 and 207 on M and B RNA respectively. By using a cloned double-stranded DNA fragment derived from near the 5' end of M RNA as a primer additional sequence from the 5' terminal region of M RNA has been obtained.

MeSH Terms
Base Sequence Cloning, Molecular Codon/genetics DNA/metabolism DNA, Viral/genetics Mosaic Viruses/genetics Oligodeoxyribonucleotides/analysis RNA, Messenger/genetics RNA, Viral/genetics
Chemicals
Codon DNA, Viral Oligodeoxyribonucleotides RNA, Messenger RNA, Viral DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lomonossoff G P
Shanks M
Matthes H D
Singh M
Gait M J
References (37)
37 references, click to expand
  1. Sequence homology adjacent to the 3' terminal poly(A) of cowpea mosaic virus RNAs.
    Nucleic Acids Res. 1979 Sep 25;7(2):493-500 PMID: 91160
  2. The use of nuclease P1 in sequence analysis of end group labeled RNA.
    Nucleic Acids Res. 1977 Dec;4(12):4091-108 PMID: 202925
  3. Sequence of 1060 3'-terminal nucleotides of poliovirus RNA as determined by a modification of the dideoxynucleotide method.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3196-200 PMID: 6158042
  4. Binding of ribosomes to the 5' leader sequence (N = 258) of RNA 3 from alfalfa mosaic virus.
    Nucleic Acids Res. 1981 Mar 11;9(5):1087-100 PMID: 7232211
  5. Nucleotide sequences adjacent to the proteins covalently linked to the cowpea mosaic virus genome. Sequence determination after labelling in vitro using RNA ligase.
    Eur J Biochem. 1979 Nov 1;101(1):45-9 PMID: 116852
  6. Polyadenylic acid at the 3'-terminus of poliovirus RNA.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1877-82 PMID: 4340162
  7. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
    Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
  8. Protein bound to the genome RNAs of cowpea mosaic virus.
    Eur J Biochem. 1978 Dec 1;92(1):45-51 PMID: 215411
  9. Polypeptide cleavages in the formation of poliovirus proteins.
    Proc Natl Acad Sci U S A. 1968 Sep;61(1):77-84 PMID: 4301595
  10. Complete nucleotide sequences of the coat protein messenger RNAs of brome mosaic virus and cowpea chlorotic mottle virus.
    Nucleic Acids Res. 1982 Jan 22;10(2):703-13 PMID: 6895941
  11. Complete nucleotide sequence of brome mosaic virus RNA3.
    J Mol Biol. 1981 Nov 25;153(1):23-38 PMID: 7338913
  12. Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditions.
    Virology. 1974 Aug;60(2):515-21 PMID: 4367491
  13. Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.
    Nucleic Acids Res. 1979 Apr;6(4):1287-308 PMID: 109810
  14. Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.
    Cell. 1978 Mar;13(3):551-63 PMID: 77738
  15. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
    Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
  16. A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.
    Nucleic Acids Res. 1978 Dec;5(12):4505-22 PMID: 745988
  17. The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp.
    Nucleic Acids Res. 1975 Jul;2(7):1189-201 PMID: 168558
  18. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  19. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  20. Evaluation of the "scanning model" for initiation of protein synthesis in eucaryotes.
    Cell. 1980 Nov;22(1 Pt 1):7-8 PMID: 7000367
  21. Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus.
    Virology. 1973 Nov;56(1):198-206 PMID: 4745624
  22. 5'-terminal structure of poliovirus polyribosomal RNA is pUp.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30 PMID: 174102
  23. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887-91 PMID: 6272282
  24. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  25. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):1015-22 PMID: 4299264
  26. Complete nucleotide sequence of alfalfa mosaic virus RNA 4.
    Nucleic Acids Res. 1980 May 24;8(10):2213-23 PMID: 7433090
  27. Relationship between structure of the 5' noncoding region of viral mRNA and efficiency in the initiation step of protein synthesis in a eukaryotic system.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1012-6 PMID: 7041111
  28. Nucleotide sequence at the 5' extremity of turnip yellow mosaic virus genome RNA.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3168-72 PMID: 16592542
  29. Nucleotide sequence of turnip yellow mosaic virus coat protein mRNA.
    Cell. 1978 Sep;15(1):113-22 PMID: 699035
  30. Nucleotide sequence at the 5' extremity of tobacco-mosaic-virus RNA. 1. The noncoding region (nucleotides 1-68).
    Eur J Biochem. 1978 Mar 15;84(2):513-9 PMID: 639800
  31. Evidence for large precursor proteins in poliovirus synthesis.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):966-71 PMID: 4296044
  32. A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2.
    Nucleic Acids Res. 1980 Apr 25;8(8):1731-43 PMID: 6253946
  33. The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80 PMID: 174106
  34. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.
    Nature. 1977 Jul 21;268(5617):208-13 PMID: 196204
  35. Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ.
    FEBS Lett. 1977 May 15;77(2):265-9 PMID: 862928
  36. Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates.
    Virology. 1979 Jul 30;96(2):463-77 PMID: 462814
  37. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.16.4861
Published
1982-08-25
Pages
4861-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320829
Subset
IM
Databases
GENBANK
J02064, J02065
External Links
PubMed source DOI: 10.1093/nar/10.16.4861 PMC Full Text View journal details
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