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PMID: 7007807 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Studies of ribosomal proteins of yeast species and their hybrids: gel electrophoresis and immunochemical cross-reactions.

Molecular & general genetics : MGG ·Vol. 179 ·No. 2 ·1980-00-00 ·Pages 273-82

Adoutte-Panvier A, Davies JE, Gritz LR, Littlewood BS

Abstract

The cytoplasmic ribosomal proteins (r-proteins) of seventeen yeast species of the genera Saccharomyces and Kluyveromyces were analyzed by one-dimensional gradient polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The electrophoretic patterns of cytoplasmic r-proteins from different species display extensive differences in both the 40S and the 60S subunit. Relatedness of species suggested by r-protein patterns correlates well with that based on DNA/DNA homology (Bicknell and Douglas 1970). Immunochemical cross-reactions and antibiotic susceptibility tests were also used to compare different species. Analyses of r-proteins from two different interspecific hybrids showed that their ribosomes were hybrid, containing r-proteins from both parents. These findings are discussed in relation to the evolution of yeast species and the regulation of expression of r-proteins in eucaryotes.

MeSH Terms
Anti-Bacterial Agents/pharmacology Ascomycota/metabolism Cross Reactions Drug Resistance, Microbial Electrophoresis, Polyacrylamide Gel Fungal Proteins/immunology,metabolism Hybridization, Genetic Ribosomal Proteins/immunology,metabolism Ribosomes/drug effects Saccharomyces cerevisiae/metabolism Species Specificity Yeasts/metabolism
Chemicals
Anti-Bacterial Agents Fungal Proteins Ribosomal Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Adoutte-Panvier A
Davies J E
Gritz L R
Littlewood B S
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425454
Published
1980-00-00
Pages
273-82
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAAA NIH HHS · AAI 12448 · United States
External Links
PubMed source DOI: 10.1007/BF00425454
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