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PMID: 7532167 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structurally variant classes of pilus appendage fibers coexpressed from Burkholderia (Pseudomonas) cepacia.

Journal of bacteriology ·Vol. 177 ·No. 4 ·1995-02-00 ·Pages 1039-52

Goldstein R, Sun L, Jiang RZ, Sajjan U, Forstner JF, Campanelli C

Abstract

One or more of five morphologically distinct classes of appendage pili were determined to be peritrichously expressed by Burkholderia (formerly Pseudomonas) cepacia isolated from disparate sources. B. cepacia-encoded cblA pilin gene hybridization-based analysis revealed that one associated class, cable (Cbl) adhesin type IIB. cepacia pili, correlates with epidemically transmitted strains from a single cystic fibrosis (CF) center. When only phenotypic assays were available, correlations between the source and the pilus type were nonetheless observed: filamentous (Fil) type IIIB. cepacia pili correlated with CF-associated nonepidemic isolates, spine (Spn) type IVB. cepacia pili correlated with clinical (non-CF) isolates, and spike (Spk) type VB. cepacia pili correlated with environmental isolates. Further, Cbl, Fil, or Spk pili typically appear as an internal framework for constitutively coexpressed, peritrichously arranged dense mats of fine, curly mesh (Msh) type IB. cepacia pili. Constitutive coexpression of dense mats of Msh type IB. cepacia pili in association with a labyrinth of either Cbl, Fil, or Spk pili suggests possible cooperative pilus interactions mediating adhesion-based colonization in the differing environments from which the strains were isolated. Despite such correlations, phylogenetic analyses indicate that with the exception of the epidemically transmitted clusters of isolates, the remaining B. cepacia strains from the other three sources exhibited an equal degree of genetic relatedness independent of origin. As previously found for Escherichia coli, this discrepancy could be accounted for by selection-driven, in vivo horizontal transfer events between distantly related members of the species B. cepacia, leading to the genetic acquisition of environmentally appropriate adhesion-based colonization pilus operons.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Burkholderia cepacia/pathogenicity,ultrastructure Cystic Fibrosis/complications,microbiology DNA, Bacterial/genetics Disease Outbreaks Electrophoresis, Gel, Pulsed-Field Fimbriae Proteins Fimbriae, Bacterial/classification,genetics,physiology,ultrastructure Genes, Bacterial/genetics Humans Microscopy, Electron Models, Genetic Negative Staining Ontario/epidemiology Pseudomonas Infections/complications,epidemiology,etiology Sequence Homology, Nucleic Acid
Chemicals
Bacterial Outer Membrane Proteins DNA, Bacterial Fimbriae Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Goldstein R
Section of Molecular Genetics, Maxwell Finland Laboratory for Infectious Diseases, Boston University School of Medicine, Massachusetts.
Sun L
Jiang R Z
Sajjan U
Forstner J F
Campanelli C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.4.1039-1052.1995
Published
1995-02-00
Pages
1039-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176700
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.4.1039-1052.1995 PMC Full Text View journal details
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