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PMID: 7537871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Splicing of a circular yeast pre-mRNA in vitro.

Nucleic acids research ·Vol. 23 ·No. 7 ·1995-04-11 ·Pages 1133-9

Schindewolf CA, Domdey H

Abstract

We have tested the fate of a circularized synthetic pre-mRNA transcript in a whole cell splicing extract of Saccharomyces cerevisiae. Our results demonstrate that this circular precursor RNA is able to induce spliceosome formation in vitro and that the products of the following splicing reaction are the lariat-shaped intron, and a mature circular mRNA. Thus, it would appear that free 5' and/or 3' ends are not obligatory for a splicing reaction to occur, although we find its efficiency to be strongly influenced by the presence or lack of free ends. To our knowledge, this is the first demonstration that a circular pre-mRNA molecule is recognized as a suitable substrate by an eukaryotic mRNA splicing apparatus.

Related Genes
ACT1
MeSH Terms
Base Sequence DNA, Fungal/genetics Electrophoresis, Gel, Two-Dimensional Genes, Fungal Molecular Sequence Data RNA/genetics,isolation & purification,metabolism RNA Precursors/genetics,isolation & purification,metabolism RNA Processing, Post-Transcriptional RNA Splicing RNA, Circular RNA, Fungal/genetics,isolation & purification,metabolism Saccharomyces cerevisiae/genetics,metabolism Spliceosomes/metabolism Transcription, Genetic
Chemicals
DNA, Fungal RNA Precursors RNA, Circular RNA, Fungal RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schindewolf C A
Institut für Biochemie, Genzentrum, Ludwig-Maximilians-Universität München, Germany.
Domdey H
References (39)
39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.7.1133
Published
1995-04-11
Pages
1133-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306821
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.7.1133 PMC Full Text View journal details
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