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PMID: 3045753 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular consequences of truncations of the first exon for in vitro splicing of yeast actin pre-mRNA.

Nucleic acids research ·Vol. 16 ·No. 15 ·1988-08-11 ·Pages 7233-9

Duchêne M, Löw A, Schweizer A, Domdey H

Abstract

A defined minimum length of the first exon is required for the generation of spliced products from a synthetic yeast actin mRNA-precursor in vitro. If the first exon is 1, 2, 3 or 5 nucleotides long, only the first step of the splicing reaction can take place. A transcript starting with the first nucleotide of the intron does not get converted into any of the normally obtained splicing products or intermediates. On the other hand, spliceosome assembly does not depend on the presence of a first exon.

MeSH Terms
Actins/genetics Exons In Vitro Techniques Molecular Weight Nucleic Acid Precursors/genetics RNA Splicing Ribonucleoproteins/physiology Saccharomyces cerevisiae/genetics Structure-Activity Relationship
Chemicals
Actins Nucleic Acid Precursors Ribonucleoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Duchêne M
Laboratorium für Molekulare Biologie, Genzentrum-der-Ludwig-Maximilians-Universität, München, Martinsried, FRG.
Löw A
Schweizer A
Domdey H
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.15.7233
Published
1988-08-11
Pages
7233-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338405
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.15.7233 PMC Full Text View journal details
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