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PMID: 7615637 Published · ppublish English Journal Article

p120, a p120-related protein (p100), and the cadherin/catenin complex.

The Journal of cell biology ·Vol. 130 ·No. 2 ·1995-07-00 ·Pages 369-81

Staddon JM, Smales C, Schulze C, Esch FS, Rubin LL

Abstract

Cadherins and catenins play an important role in cell-cell adhesion. Two of the catenins, beta and gamma, are members of a group of proteins that contains a repeating amino acid motif originally described for the Drosophila segment polarity gene armadillo. Another member of this group is a 120-kD protein termed p120, originally identified as a substrate of the tyrosine kinase pp60src. In this paper, we show that endothelial and epithelial cells express p120 and p100, a 100-kD, p120-related protein. Peptide sequencing of p100 establishes it as highly related to p120. p120 and p100 both appear associated with the cadherin/catenin complex, but independent p120/catenin and p100/catenin complexes can be isolated. This association is shown by coimmunoprecipitation of cadherins and catenins with an anti-p120/p100 antibody, and of p120/p100 with cadherin or catenin antibodies. Immunocytochemical analysis with a p120-specific antibody reveals junctional colocalization of p120 and beta-catenin in epithelial cells. Catenins and p120/p100 also colocalize in endothelial and epithelial cells in culture and in tissue sections. The cellular content of p120/p100 and beta-catenin is similar in MDCK cells, but only approximately 20% of the p120/p100 pool associates with the cadherin/catenin complex. Our data provide further evidence for interactions among the different arm proteins and suggest that p120/p100 may participate in regulating the function of cadherins and, thereby, other processes influenced by cell-cell adhesion.

MeSH Terms
Amino Acid Sequence Animals Cadherins/analysis,metabolism Catenins Cell Adhesion Molecules/analysis,metabolism Cell Line Cells, Cultured Cytoskeletal Proteins/analysis,metabolism Endothelium/chemistry,cytology Endothelium, Vascular/chemistry,cytology Epithelial Cells Epithelium/chemistry Humans Immunoblotting Immunohistochemistry Intercellular Junctions/chemistry Molecular Sequence Data Phosphoproteins/analysis,metabolism Precipitin Tests Trans-Activators Tumor Cells, Cultured beta Catenin
Chemicals
CTNNB1 protein, human Cadherins Catenins Cell Adhesion Molecules Cytoskeletal Proteins Phosphoproteins Trans-Activators beta Catenin delta catenin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Staddon J M
Eisai London Research Laboratories Ltd., University College London, United Kingdom.
Smales C
Schulze C
Esch F S
Rubin L L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.130.2.369
Published
1995-07-00
Pages
369-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2199935
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.130.2.369 PMC Full Text View journal details
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