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PMID: 7621829 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

BS69, a novel adenovirus E1A-associated protein that inhibits E1A transactivation.

The EMBO journal ·Vol. 14 ·No. 13 ·1995-07-03 ·Pages 3159-69

Hateboer G, Gennissen A, Ramos YF, Kerkhoven RM, Sonntag-Buck V, Stunnenberg HG, Bernards R

Abstract

The adenovirus E1A gene products are nuclear phosphoproteins that can transactivate the other adenovirus early genes as well as several cellular genes, and can transform primary rodent cells in culture. Transformation and transactivation by E1A proteins is most likely to be mediated through binding to several cellular proteins, including the retinoblastoma gene product pRb, the pRb-related p107 and p130, and the TATA box binding protein TBP. We report here the cloning of BS69, a novel protein that specifically interacts with adenovirus 5 E1A. BS69 has no significant homology to known proteins and requires the region that is unique to the large (289R) E1A protein for high affinity binding. BS69 and E1A proteins coimmunoprecipitate in adenovirus-transformed 293 cells, indicating that these proteins also interact in vivo. BS69 specifically inhibits transactivation by the 289R E1A protein, but not by the 243R E1A protein. BS69 also suppressed the E1A-stimulated transcription of the retinoic acid receptor in COS cells, but did not affect the cellular E1A-like activity that is present in embryonic carcinoma cells. Our data indicate that BS69 is a novel and specific suppressor of E1A-activated transcription.

MeSH Terms
Adenoviridae Adenovirus E1A Proteins/biosynthesis,genetics Amino Acid Sequence Animals Base Sequence Binding Sites Carrier Proteins/genetics,metabolism Cell Cycle Proteins Chromosome Mapping Co-Repressor Proteins DNA-Binding Proteins Gene Expression Regulation, Neoplastic Humans Mice Molecular Sequence Data Receptors, Retinoic Acid/genetics,metabolism Transcription, Genetic Transcriptional Activation Tretinoin/metabolism Tumor Cells, Cultured
Chemicals
Adenovirus E1A Proteins Carrier Proteins Cell Cycle Proteins Co-Repressor Proteins DNA-Binding Proteins Receptors, Retinoic Acid ZMYND11 protein, human Zmynd11 protein, mouse Tretinoin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hateboer G
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Amsterdam.
Gennissen A
Ramos Y F
Kerkhoven R M
Sonntag-Buck V
Stunnenberg H G
Bernards R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-07-03
Pages
3159-69
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394377
Subset
IM
Databases
GENBANK
X86098
External Links
PubMed source PMC Full Text View journal details
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