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PMID: 7651391 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Epstein-Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE.

Molecular and cellular biology ·Vol. 15 ·No. 9 ·1995-09-00 ·Pages 4735-44

Tong X, Drapkin R, Yalamanchili R, Mosialos G, Kieff E

Abstract

Epstein-Barr virus nuclear antigen 2 (EBNA 2) activates transcription of specific genes and is essential for B-lymphocyte transformation. EBNA 2 has an acidic activation domain which interacts with general transcription factors TFIIB, TFIIH, and TAF40. We now show that EBNA 2 is specifically bound to a novel nuclear protein, p100, and that p100 can coactivate gene expression mediated by the EBNA 2 acidic domain. The EBNA 2 acidic domain was used to affinity purify p100. cDNA clones encoding the p100 open reading frame were identified on the basis of peptide sequences of the purified protein. Antibody against p100 coimmunoprecipitated p100 and EBNA 2 from Epstein-Barr virus-transformed lymphocyte extracts, indicating that EBNA 2 and p100 are complexed in vivo. p100 overexpression in cells specifically augmented EBNA 2 acidic domain-mediated activation. The coactivating effect is probably mediated by p100 interaction with TFIIE. Bacterially expressed p100 specifically adsorbs TFIIE from nuclear extracts, and in vitro-translated p56 or p34 TFIIE subunit can independently bind to p100. p100 also appears to be essential for normal cell growth, since cell viability was reduced by antisense p100 RNA and restored by sense p100 RNA expression.

MeSH Terms
Amino Acid Sequence Antigens, Viral/genetics,metabolism Antisense Elements (Genetics) B-Lymphocytes/metabolism Base Sequence Cell Compartmentation Cells, Cultured Cloning, Molecular DNA-Binding Proteins/genetics,metabolism Endonucleases Epstein-Barr Virus Nuclear Antigens Fluorescent Antibody Technique Genes, Lethal/genetics Humans Immunoblotting Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Nuclear Proteins/genetics,metabolism Peptide Fragments/genetics,metabolism Precipitin Tests Protein Binding Sequence Analysis Transcription Factors/metabolism Transcription Factors, TFII Transcriptional Activation
Chemicals
Antigens, Viral Antisense Elements (Genetics) DNA-Binding Proteins Epstein-Barr Virus Nuclear Antigens Nuclear Proteins Peptide Fragments Transcription Factors Transcription Factors, TFII transcription factor TFIIE Endonucleases SND1 protein, human
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tong X
Department of Medicine, Harvard University, Boston, Massachusetts 02115, USA.
Drapkin R
Yalamanchili R
Mosialos G
Kieff E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.9.4735
Published
1995-09-00
Pages
4735-44
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230717
Subset
IM
Grants
NIAID NIH HHS · AI08548-02 · United States
NIGMS NIH HHS · GM08360 · United States
NCI NIH HHS · R35CA47006-08 · United States
Databases
GENBANK
U22055
External Links
PubMed source DOI: 10.1128/MCB.15.9.4735 PMC Full Text View journal details
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