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PMID: 7705652 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Extra telomeres, but not internal tracts of telomeric DNA, reduce transcriptional repression at Saccharomyces telomeres.

Genetics ·Vol. 139 ·No. 1 ·1995-01-00 ·Pages 67-79

Wiley EA, Zakian VA

Abstract

Yeast telomeric DNA is assembled into a nonnucleosomal chromatin structure known as the telosome, which is thought to influence the transcriptional repression of genes placed in its vicinity, a phenomenon called telomere position effect (TPE). The product of the RAP1 gene, Rap1p, is a component of the telosome. We show that the fraction of cells exhibiting TPE can be substantially reduced by expressing large amounts of a deletion derivative of Rap1p that is unable to bind DNA, called Rap1 delta BBp, or by introducing extra telomeres on a linear plasmid, presumably because both compete in trans with telomeric chromatin for factor(s) important for TPE. This reduction in TPE, observed in three different strains, was demonstrated for two different genes, each assayed at a different telomere. In contrast, the addition of internal tracts of telomeric DNA on a circular plasmid had very little effect on TPE. The product of the SIR3 gene, Sir3p, appears to be limiting for TPE. Overexpression of Sir3p completely suppressed the reduction in TPE observed with expression of Rap1 delta BBp, but did not restore high levels of TPE to cells with extra telomeres. These results suggest that extra telomeres must titrate a factor other than Sir3p that is important for TPE. These results also provide evidence for a terminus-specific binding factor that is a factor with a higher affinity for DNA termini than for nonterminal tracts of telomeric DNA and indicate that this factor is important for TPE.

MeSH Terms
DNA, Fungal/genetics DNA-Binding Proteins/genetics Fungal Proteins/biosynthesis GTP-Binding Proteins/biosynthesis Gene Expression Regulation, Fungal Repressor Proteins/genetics Saccharomyces/genetics Saccharomyces cerevisiae Proteins Silent Information Regulator Proteins, Saccharomyces cerevisiae Species Specificity Telomere/genetics Telomere-Binding Proteins Trans-Activators/biosynthesis Transcription, Genetic rap GTP-Binding Proteins
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Repressor Proteins SIR3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Silent Information Regulator Proteins, Saccharomyces cerevisiae Telomere-Binding Proteins Trans-Activators RIF1 protein, S cerevisiae GTP-Binding Proteins rap GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wiley E A
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Zakian V A
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
Published
1995-01-00
Pages
67-79
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206349
Subset
IM
Grants
NIA NIH HHS · AG-00057 · United States
NIGMS NIH HHS · GM-43265 · United States
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