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PMID: 7777503 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA-mediated immunization to the hepatitis B surface antigen in mice: aspects of the humoral response mimic hepatitis B viral infection in humans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 12 ·1995-06-06 ·Pages 5307-11

Michel ML, Davis HL, Schleef M, Mancini M, Tiollais P, Whalen RG

Abstract

Intramuscular injection of plasmid DNA expression vectors encoding the three envelope proteins of the hepatitis B virus (HBV) induced humoral responses in C57BL/6 mice specific to several antigenic determinants of the viral envelope. The first antibodies appeared within 1-2 weeks after injection of DNA and included antibodies of the IgM isotype. Over the next few weeks, an IgM to IgG class switch occurred, indicating helper T-lymphocyte activity. Peak IgG titers were reached by 4-8 weeks after a single DNA injection and were maintained for at least 6 months without further DNA injections. The antibodies to the envelope proteins reacted with group- and subtype-specific antigenic determinants of the HBV surface antigen (HBsAg). Expression vectors encoding the major (S) and middle (preS2 plus S) envelope proteins induced antibodies specific to the S protein and preS2 domain, and preS2 antibodies were prominent at early time points. In general, the expression vectors induced humoral responses in mice that mimic those observed in humans during the course of natural HBV infection.

MeSH Terms
Animals Antibodies, Viral/biosynthesis Cell Line DNA, Recombinant/genetics,immunology DNA, Viral/genetics,immunology Genetic Vectors Hepatitis B/immunology Hepatitis B Surface Antigens/genetics,immunology Humans Immunization Male Mice Mice, Inbred C57BL Plasmids Protein Precursors/genetics,immunology Transfection Viral Envelope Proteins/genetics,immunology
Chemicals
Antibodies, Viral DNA, Recombinant DNA, Viral Hepatitis B Surface Antigens Protein Precursors Viral Envelope Proteins presurface protein 2, hepatitis B surface antigen
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Michel M L
Unité de Recombinaison et Expression Génétique, Institut Pasteur, France.
Davis H L
Schleef M
Mancini M
Tiollais P
Whalen R G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.12.5307
Published
1995-06-06
Pages
5307-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41683
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.12.5307 PMC Full Text View journal details
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