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PMID: 7806582 Published · ppublish English Journal Article

E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton.

The Journal of cell biology ·Vol. 127 ·No. 6 Pt 2 ·1994-12-00 ·Pages 2061-9

Hülsken J, Birchmeier W, Behrens J

Abstract

beta-Catenin is involved in the formation of adherens junctions of mammalian epithelia. It interacts with the cell adhesion molecule E-cadherin and also with the tumor suppressor gene product APC, and the Drosophila homologue of beta-catenin, armadillo, mediates morphogenetic signals. We demonstrate here that E-cadherin and APC directly compete for binding to the internal, armadillo-like repeats of beta-catenin; the NH2-terminal domain of beta-catenin mediates the interaction of the alternative E-cadherin and APC complexes to the cytoskeleton by binding to alpha-catenin. Plakoglobin (gamma-catenin), which is structurally related to beta-catenin, mediates identical interactions. We thus show that the APC tumor suppressor gene product forms strikingly similar associations as found in cell junctions and suggest that beta-catenin and plakoglobin are central regulators of cell adhesion, cytoskeletal interaction, and tumor suppression.

MeSH Terms
Adenomatous Polyposis Coli Protein Amino Acid Sequence Base Sequence Cadherins/genetics,isolation & purification,metabolism Cells, Cultured Cytoskeletal Proteins/genetics,isolation & purification,metabolism Cytoskeleton/metabolism Desmoplakins Fluorescent Antibody Technique Intercellular Junctions Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Precipitin Tests Protein Binding Recombinant Proteins/metabolism Trans-Activators alpha Catenin beta Catenin gamma Catenin
Chemicals
Adenomatous Polyposis Coli Protein Cadherins Cytoskeletal Proteins Desmoplakins Recombinant Proteins Trans-Activators alpha Catenin beta Catenin gamma Catenin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hülsken J
Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
Birchmeier W
Behrens J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.6.2061
Published
1994-12-00
Pages
2061-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120290
Subset
IM
Databases
GENBANK
X87838, Z19054
External Links
PubMed source DOI: 10.1083/jcb.127.6.2061 PMC Full Text View journal details
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