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PMID: 7853521 Published · ppublish English Case Reports Comparative Study Journal Article Research Support, Non-U.S. Gov't

Syncytium-inducing (SI) phenotype suppression at seroconversion after intramuscular inoculation of a non-syncytium-inducing/SI phenotypically mixed human immunodeficiency virus population.

Journal of virology ·Vol. 69 ·No. 3 ·1995-03-00 ·Pages 1810-8

Cornelissen M, Mulder-Kampinga G, Veenstra J, Zorgdrager F, Kuiken C, Hartman S, Dekker J, van der Hoek L, Sol C, Coutinho R

Abstract

Two distinct biological phenotypes of human immunodeficiency virus (HIV) have been described: the non-syncytium-inducing (NSI) phenotype, best characterized by the inability to infect MT-2 cells, and the syncytium-inducing (SI) phenotype, with the ability to infect MT-2 cells. The earliest virus population observed following HIV transmission is generally of the NSI phenotype, even after exposure to inocula of mixed NSI/SI phenotype. In this study, the issue of intrapatient selection of virus phenotype following transmission was addressed by studying two cases of accidental transmission. A comparison of the sequences of the V1-V2 and the V3 coding regions of the envelope gene and the p17 region of the gag gene showed that the donor-recipient pairs were tightly clustered in all gene segments, but away from local and published transmission controls. The intrasample variation of the p17 sequence was greater in the recipients and smaller in the donors than that of the V3 region sequence, indicating selection of V3 at transmission. In these transmission cases, the effects of an intravenous inoculation of a small quantity of blood containing predominantly SI V3 sequences (6 of 8 clonal sequences) were compared with those of an intramuscular inoculation of a large quantity of blood containing predominantly NSI viruses (14 of 16 clonal sequences). Both SI and NSI V3 regions were demonstrated to be phenotypic expressions of genetically related viral strains. The inoculation of the predominantly SI virus population resulted in the persistence of an SI virus population in the recipient and a rapid CD4+ T-cell decline. The inoculation of the predominantly NSI population resulted in a selective amplification of SI viruses before seroconversion, followed by a suppression of SI viruses at seroconversion and a rapid decline of CD4+ T-cell numbers. These data suggest that the suppression of SI viruses can be accomplished following the development of HIV-specific immunity and that the ability to suppress SI viruses does not prevent the development of immunodeficiency.

Related Genes
env gag
MeSH Terms
Acquired Immunodeficiency Syndrome/transmission Adult Amino Acid Sequence Base Sequence Cell Fusion Consensus Sequence DNA Primers/chemistry Female Genes, env Genes, gag Genetic Variation HIV Envelope Protein gp120/genetics HIV Seropositivity/microbiology HIV-1/genetics,pathogenicity Humans Molecular Sequence Data Phylogeny RNA, Viral/genetics Sequence Alignment Sequence Homology, Amino Acid Time Factors
Chemicals
DNA Primers HIV Envelope Protein gp120 RNA, Viral
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Cornelissen M
Human Retrovirus Laboratory, Academic Medical Centre, Amsterdam, The Netherlands.
Mulder-Kampinga G
Veenstra J
Zorgdrager F
Kuiken C
Hartman S
Dekker J
van der Hoek L
Sol C
Coutinho R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.3.1810-1818.1995
Published
1995-03-00
Pages
1810-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC188790
Subset
IM
Databases
GENBANK
Z47411, Z47412, Z47413, Z47414, Z47415, Z47416, Z47417, Z47418, Z47419, Z47420, Z47421, Z47422, Z47423, Z47424, Z47425, Z47426, Z47427, Z47428, Z47429, Z47430, Z47431, Z47432, Z47433, Z47434, Z47435, Z47436, Z47437, Z47438, Z47439, Z47440
External Links
PubMed source DOI: 10.1128/JVI.69.3.1810-1818.1995 PMC Full Text View journal details
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