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PMID: 7905824 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic and immunochemical evidence for CD4-dependent association of p56lck with the alpha beta T-cell receptor (TCR): regulation of TCR-induced activation.

The EMBO journal ·Vol. 13 ·No. 1 ·1994-01-01 ·Pages 90-9

Díez-Orejas R, Ballester S, Feito MJ, Ojeda G, Criado G, Ronda M, Portolés P, Rojo JM

Abstract

Recent observations suggest that the tyrosine kinase p56lck is involved in the transduction of transmembrane signals through the antigen specific T cell receptor (TCR) in CD4+ T cells. By means of in vitro kinase assays, we have found that p56lck coprecipitated with the TCR from lysates of a murine CD4+ T cell line in the absence of TCR-mediated stimuli. Analysis of CD4- mutants and CD4-transfected cells shows that p56lck-TCR association occurred only when CD4 was present. The functional importance of CD4:p56lck-TCR association was demonstrated by low activating potential of rare clonotypic antibodies which did not coprecipitate CD4:p56lck, as well as by total or partial loss of anti-TCR or antigen induced stimulation in CD4- cells, which could be recovered by CD4 transfection. Complementation assays using different anti-TCR antibodies suggest that cross linking of TCR-p56lck:CD4 plus structural changes in the complex are needed for efficient transduction of activating signals through the TCR in these cells.

MeSH Terms
Animals CD3 Complex/metabolism CD4 Antigens/genetics,metabolism CD4-Positive T-Lymphocytes/immunology,metabolism Cell Line Flow Cytometry Lymphocyte Activation/physiology Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Mice Mice, Inbred C3H Mutation Precipitin Tests Protein-Tyrosine Kinases/metabolism Receptors, Antigen, T-Cell, alpha-beta/metabolism Signal Transduction Transfection
Chemicals
CD3 Complex CD4 Antigens Receptors, Antigen, T-Cell, alpha-beta Protein-Tyrosine Kinases Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Díez-Orejas R
Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.
Ballester S
Feito M J
Ojeda G
Criado G
Ronda M
Portolés P
Rojo J M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-01-01
Pages
90-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394782
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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