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PMID: 7937919 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of NF-E2-related factor 2 (Nrf2), a NF-E2-like basic leucine zipper transcriptional activator that binds to the tandem NF-E2/AP1 repeat of the beta-globin locus control region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 21 ·1994-10-11 ·Pages 9926-30

Moi P, Chan K, Asunis I, Cao A, Kan YW

Abstract

Hypersensitive site 2 located in the beta-globin locus control region confers high levels of expression to the genes of the beta-globin cluster. A tandem repeat of the consensus sequence for the transcription factors AP1 and NF-E2 (activating protein 1 and nuclear factor erythroid 2, respectively) is present within hypersensitive site 2 and is absolutely required for strong enhancer activity. This sequence binds, in vitro and in vivo, to ubiquitous proteins of the AP1 family and to the recently cloned erythroid-specific transcription factor NF-E2. Using the tandem repeat as a recognition site probe to screen a lambda gt11 cDNA expression library from K562 cells, we isolated several DNA binding proteins. Here, we report the characterization of one of the clones isolated. The gene, which we named Nrf2 (NF-E2-related factor 2), is encoded within a 2.2-kb transcript and predicts a 66-kDa protein with a basic leucine zipper DNA binding domain highly homologous to that of NF-E2. Although Nrf2 is expressed ubiquitously, a role of this protein in mediating enhancer activity of hypersensitive site 2 in erythroid cells cannot be excluded. In this respect, Nrf2 contains a powerful acidic activation domain that may participate in the transcriptional stimulation of beta-globin genes.

Related Genes
NF-E2 Nrf2
MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites Blotting, Northern Cell Line Consensus Sequence DNA-Binding Proteins/genetics,isolation & purification,metabolism Erythroid-Specific DNA-Binding Factors Gene Expression Gene Library Genes, Regulator Globins/genetics Humans Leucine Zippers Leukemia, Erythroblastic, Acute Molecular Sequence Data Multigene Family NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit NF-E2-Related Factor 2 Oligodeoxyribonucleotides Open Reading Frames RNA, Messenger/isolation & purification,metabolism Repetitive Sequences, Nucleic Acid Sequence Homology, Amino Acid Trans-Activators/genetics,isolation & purification,metabolism Transcription Factor AP-1/metabolism Transcription Factors/genetics,metabolism Tumor Cells, Cultured Zinc Fingers
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit NF-E2-Related Factor 2 NFE2 protein, human NFE2L2 protein, human Oligodeoxyribonucleotides RNA, Messenger Trans-Activators Transcription Factor AP-1 Transcription Factors Globins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moi P
Istituto di Clinica e Biologia dell' Età Evolutiva, Università di Cagliari, Italy.
Chan K
Asunis I
Cao A
Kan Y W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.21.9926
Published
1994-10-11
Pages
9926-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44930
Subset
IM
Grants
NIDDK NIH HHS · DK16666 · United States
Databases
GENBANK
S74017
External Links
PubMed source DOI: 10.1073/pnas.91.21.9926 PMC Full Text View journal details
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