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PMID: 8248256 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning of Nrf1, an NF-E2-related transcription factor, by genetic selection in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 23 ·1993-12-01 ·Pages 11371-5

Chan JY, Han XL, Kan YW

Abstract

We have devised a complementation assay in yeast to clone mammalian transcriptional activators and have used it to identify a human basic leucine-zipper transcription factor that we have designated Nrf1 for NF-E2-related factor 1. Nrf1 potentially encodes a 742-aa protein and displays marked homology to the mouse and human NF-E2 transcription factors. Nrf1 activates transcription via NF-E2 binding sites in yeast cells. The ubiquitous expression pattern of Nrf1 and the range of promoters containing the NF-E2 binding motif suggest that this gene may play a role in the regulation of heme synthesis and ferritin genes.

Related Genes
CNC Fos JUN B JUN D NFE2 Nrf1 cJUN
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Complementary/genetics DNA-Binding Proteins/genetics Erythropoiesis Gene Expression Genetic Complementation Test Humans Leucine Zippers Molecular Sequence Data NF-E2-Related Factor 1 Nuclear Respiratory Factor 1 Nuclear Respiratory Factors Oligodeoxyribonucleotides/chemistry RNA, Messenger/genetics Saccharomyces cerevisiae Sequence Alignment Sequence Homology, Amino Acid Tissue Distribution Trans-Activators Transcription Factors/genetics Transcription, Genetic
Chemicals
DNA, Complementary DNA-Binding Proteins NF-E2-Related Factor 1 NRF1 protein, human Nuclear Respiratory Factor 1 Nuclear Respiratory Factors Oligodeoxyribonucleotides RNA, Messenger Trans-Activators Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chan J Y
Department of Laboratory Medicine, University of California, San Francisco 94143-0724.
Han X L
Kan Y W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.23.11371
Published
1993-12-01
Pages
11371-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47984
Subset
IM
Grants
NIDDK NIH HHS · DK16666 · United States
Databases
GENBANK
L24123
External Links
PubMed source DOI: 10.1073/pnas.90.23.11371 PMC Full Text View journal details
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