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PMID: 8248255 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of cDNA encoding the human NF-E2 protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 23 ·1993-12-01 ·Pages 11366-70

Chan JY, Han XL, Kan YW

Abstract

The human homolog of mouse NF-E2 was isolated from the K562 cell line and found to encode a member of the basic leucine-zipper family of DNA-binding regulatory proteins. The deduced amino acid sequence of the mouse and human proteins exhibited near identity. Comparison to the related protein, Nrf1, revealed significant homologies at isolated regions, particularly within the basic domain, suggesting that NF-E2 and Nrf1 are members of a distinct subfamily of basic leucine-zipper proteins that share similar DNA-binding properties. High levels of human NF-E2 mRNA were observed in human erythroleukemic cell lines examined. Extensive survey of human tissue samples found NF-E2 expression not limited to erythropoeitic organs. Expression in the colon and testis suggests that NF-E2 may participate in the regulation of genes other than globin.

Related Genes
CNC NFE2 Nrf1 cFos cJun
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Complementary/genetics DNA-Binding Proteins/genetics Erythroid-Specific DNA-Binding Factors Erythropoiesis Gene Expression Humans Leucine Zippers Mice Molecular Sequence Data NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Oligodeoxyribonucleotides/chemistry RNA, Messenger/genetics Sequence Alignment Sequence Homology, Amino Acid Tissue Distribution Transcription Factors/genetics
Chemicals
DNA, Complementary DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit NFE2 protein, human Nfe2 protein, mouse Oligodeoxyribonucleotides RNA, Messenger Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chan J Y
Department of Laboratory Medicine, University of California, San Francisco 94143-0724.
Han X L
Kan Y W
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.23.11366
Published
1993-12-01
Pages
11366-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47983
Subset
IM
Grants
NIDDK NIH HHS · DK 16666 · United States
Databases
GENBANK
L24122
External Links
PubMed source DOI: 10.1073/pnas.90.23.11366 PMC Full Text View journal details
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