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PMID: 8034744 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Determination of the alpha-actinin-binding site on actin filaments by cryoelectron microscopy and image analysis.

The Journal of cell biology ·Vol. 126 ·No. 2 ·1994-07-00 ·Pages 433-43

McGough A, Way M, DeRosier D

Abstract

The three-dimensional structure of actin filaments decorated with the actin-binding domain of chick smooth muscle alpha-actinin (alpha A1-2) has been determined to 21-A resolution. The shape and location of alpha A1-2 was determined by subtracting maps of F-actin from the reconstruction of decorated filaments. alpha A1-2 resembles a bell that measures approximately 38 A at its base and extends 42 A from its base to its tip. In decorated filaments, the base of alpha A1-2 is centered about the outer face of subdomain 2 of actin and contacts subdomain 1 of two neighboring monomers along the long-pitch (two-start) helical strands. Using the atomic model of F-actin (Lorenz, M., D. Popp, and K. C. Holmes. 1993. J. Mol. Biol. 234:826-836.), we have been able to test directly the likelihood that specific actin residues, which have been previously identified by others, interact with alpha A1-2. Our results indicate that residues 86-117 and 350-375 comprise distinct binding sites for alpha-actinin on adjacent actin monomers.

MeSH Terms
Actin Cytoskeleton/chemistry,ultrastructure Actinin/chemistry,metabolism,ultrastructure Actins/chemistry,metabolism,ultrastructure Animals Binding Sites Chickens Computer Graphics Fourier Analysis Image Processing, Computer-Assisted/methods Microscopy, Electron/methods Molecular Structure Muscles/chemistry Protein Conformation
Chemicals
Actins Actinin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McGough A
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254.
Way M
DeRosier D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.126.2.433
Published
1994-07-00
Pages
433-43
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200043
Subset
IM
Grants
NIAMS NIH HHS · AR08207-03 · United States
NCI NIH HHS · CA44704 · United States
NIGMS NIH HHS · GM26357 · United States
External Links
PubMed source DOI: 10.1083/jcb.126.2.433 PMC Full Text View journal details
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