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PMID: 8071223 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification of VCR, a repeated sequence associated with a locus encoding a hemagglutinin in Vibrio cholerae O1.

Journal of bacteriology ·Vol. 176 ·No. 17 ·1994-09-00 ·Pages 5450-8

Barker A, Clark CA, Manning PA

Abstract

We have determined the nucleotide sequence of a 6.3-kb BamHI fragment of the chromosome of Vibrio cholerae 569B that includes the sequence of the mannose-fucose-resistant hemagglutinin reported previously (V.L. Franzon, A. Barker, and P. A. Manning, Infect. Immun. 61:3032-3037, 1993). This region contains nine copies of a 124-bp direct repeat, here named VCR, of imperfect dyad symmetry, that are shown by Southern hybridization to occur at least 60 to 100 times in the V. cholerae O1 chromosome. Large-scale chromosomal mapping suggests that the repeats are confined to about 10% of the chromosome. Related sequences are also found in non-O1 V. cholerae but not in other members of the family Vibrionaceae. However, VCR is unrelated to other previously described repetitive sequences.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA, Bacterial/genetics,isolation & purification Escherichia coli Hemagglutinins/biosynthesis,genetics Molecular Sequence Data Nucleic Acid Conformation Oligonucleotide Probes Open Reading Frames Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Serotyping Vibrio/classification,genetics Vibrio cholerae/classification,genetics,metabolism
Chemicals
DNA, Bacterial Hemagglutinins Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barker A
Department of Microbiology and Immunology, University of Adelaide, Australia.
Clark C A
Manning P A
References (47)
47 references, click to expand
  1. Repetitive extragenic palindromic sequences, mRNA stability and gene expression: evolution by gene conversion? A review.
    Gene. 1988 Dec 10;72(1-2):3-14 PMID: 3072249
  2. DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8850-4 PMID: 2848243
  3. Dysfunction of chromosomal loop attachment sites: illegitimate recombination linked to matrix association regions and topoisomerase II.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5497-501 PMID: 2546156
  4. Tandem chromosomal duplications: role of REP sequences in the recombination event at the join-point.
    EMBO J. 1990 Mar;9(3):939-46 PMID: 2178927
  5. Protein:DNA interactions at chromosomal loop attachment sites.
    Genome. 1989;31(2):503-9 PMID: 2561108
  6. DNA polymerase I and a protein complex bind specifically to E. coli palindromic unit highly repetitive DNA: implications for bacterial chromosome organization.
    Nucleic Acids Res. 1990 Jul 11;18(13):3941-52 PMID: 2197600
  7. Deletions of chromosomal regions coding for fimbriae and hemolysins occur in vitro and in vivo in various extraintestinal Escherichia coli isolates.
    Microb Pathog. 1990 Mar;8(3):213-25 PMID: 1974320
  8. Genetic instability and hypervariability in Streptomyces ambofaciens: towards an understanding of a mechanism of genome plasticity.
    Mol Microbiol. 1990 May;4(5):707-14 PMID: 2201867
  9. Transcription regulates oxolinic acid-induced DNA gyrase cleavage at specific sites on the E. coli chromosome.
    Nucleic Acids Res. 1990 Dec 25;18(24):7389-96 PMID: 2175434
  10. ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium and other enterobacteria.
    Mol Microbiol. 1991 Apr;5(4):825-34 PMID: 1713281
  11. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.
    Nucleic Acids Res. 1991 Dec 25;19(24):6823-31 PMID: 1762913
  12. Site-specific deletion and rearrangement of integron insert genes catalyzed by the integron DNA integrase.
    J Bacteriol. 1992 Mar;174(5):1574-85 PMID: 1311297
  13. Distinguishing between the extracellular DNases of Vibrio cholerae and development of a transformation system.
    Mol Microbiol. 1991 Oct;5(10):2547-55 PMID: 1791765
  14. Short, interspersed repetitive DNA sequences in prokaryotic genomes.
    J Bacteriol. 1992 Jul;174(14):4525-9 PMID: 1624445
  15. Physical mapping of repetitive extragenic palindromic sequences in Escherichia coli and phylogenetic distribution among Escherichia coli strains and other enteric bacteria.
    J Bacteriol. 1992 Jul;174(14):4583-93 PMID: 1624447
  16. Loss of the pigmentation phenotype in Yersinia pestis is due to the spontaneous deletion of 102 kb of chromosomal DNA which is flanked by a repetitive element.
    Mol Microbiol. 1992 Sep;6(18):2693-704 PMID: 1447977
  17. CTX genetic element encodes a site-specific recombination system and an intestinal colonization factor.
    Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3750-4 PMID: 8475125
  18. Pilus and nonpilus bacterial adhesins: assembly and function in cell recognition.
    Cell. 1993 Jun 4;73(5):887-901 PMID: 8098994
  19. Nucleotide sequence encoding the mannose-fucose-resistant hemagglutinin of Vibrio cholerae O1 and construction of a mutant.
    Infect Immun. 1993 Jul;61(7):3032-7 PMID: 8514410
  20. Specific interaction of IHF with RIBs, a class of bacterial repetitive DNA elements located at the 3' end of transcription units.
    EMBO J. 1993 Dec 15;12(13):5019-27 PMID: 8262044
  21. Integration host factor binds to a unique class of complex repetitive extragenic DNA sequences in Escherichia coli.
    Mol Microbiol. 1993 Oct;10(1):113-22 PMID: 7968507
  22. Surface-associated and soluble components of Vibrio cholerae involved in bacteria-host interactions.
    Curr Top Microbiol Immunol. 1994;192:265-81 PMID: 7859509
  23. Distribution of cholera organisms in experimental Vibrio cholerae infections: proposed mechanisms of pathogenesis and antibacterial immunity.
    Infect Immun. 1976 Jan;13(1):195-203 PMID: 1248870
  24. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  25. Characterization and distribution of the hemagglutinins produced by Vibrio cholerae.
    Infect Immun. 1982 Apr;36(1):209-14 PMID: 7076294
  26. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  27. Homology is not required for recombination mediated by DNA gyrase of Escherichia coli.
    Mol Gen Genet. 1984;193(2):238-43 PMID: 6319962
  28. Repetitive extragenic palindromic sequences: a major component of the bacterial genome.
    Cell. 1984 Jul;37(3):1015-26 PMID: 6378385
  29. A family of dispersed repetitive extragenic palindromic DNA sequences in E. coli.
    EMBO J. 1984 Jun;3(6):1417-21 PMID: 6378622
  30. Cholera epidemiology in developed and developing countries: new thoughts on transmission, seasonality, and control.
    Lancet. 1985 Feb 2;1(8423):261-2 PMID: 2857326
  31. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8 PMID: 3856838
  32. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  33. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  34. Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene.
    EMBO J. 1985 Apr;4(4):1059-66 PMID: 2990907
  35. Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1256-60 PMID: 3006041
  36. Molecular cloning and expression in Escherichia coli K-12 of the gene for a hemagglutinin from Vibrio cholerae.
    Infect Immun. 1986 Apr;52(1):279-84 PMID: 3007362
  37. Molecular cloning and expression in Escherichia coli K-12 of the O antigens of the Inaba and Ogawa serotypes of the Vibrio cholerae O1 lipopolysaccharides and their potential for vaccine development.
    Infect Immun. 1986 Aug;53(2):272-7 PMID: 2426198
  38. Cholera.
    Clin Gastroenterol. 1986 Jul;15(3):507-28 PMID: 3527493
  39. Molecular cloning of a gene coding for a Vibrio cholerae haemagglutinin.
    J Gen Microbiol. 1986 Aug;132(8):2225-34 PMID: 3794648
  40. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2833-7 PMID: 2883655
  41. Involvement of cell envelope components in the pathogenesis of Vibrio cholerae: targets for cholera vaccine development.
    Vaccine. 1987 Jun;5(2):83-7 PMID: 2885982
  42. Species specificity of bacterial palindromic units.
    J Mol Evol. 1987;25(4):371-3 PMID: 3118050
  43. A subfamily of E. coli palindromic units implicated in transcription termination?
    Ann Inst Pasteur Microbiol. 1986 Nov-Dec;137B(3):259-70 PMID: 3318868
  44. Purification, specific fragmentation, and separation of large DNA molecules.
    Methods Enzymol. 1987;155:449-67 PMID: 3431468
  45. Functional role of a highly repetitive DNA sequence in anchorage of the mouse genome.
    Nucleic Acids Res. 1988 Sep 12;16(17):8351-60 PMID: 3419921
  46. Toxin, toxin-coregulated pili, and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans.
    J Exp Med. 1988 Oct 1;168(4):1487-92 PMID: 2902187
  47. Fast and sensitive multiple sequence alignments on a microcomputer.
    Comput Appl Biosci. 1989 Apr;5(2):151-3 PMID: 2720464
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.17.5450-5458.1994
Published
1994-09-00
Pages
5450-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196733
Subset
IM
Databases
GENBANK
X64097
External Links
PubMed source DOI: 10.1128/jb.176.17.5450-5458.1994 PMC Full Text View journal details
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