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PMID: 8144463 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Genetics of the serine cycle in Methylobacterium extorquens AM1: identification of sgaA and mtdA and sequences of sgaA, hprA, and mtdA.

Journal of bacteriology ·Vol. 176 ·No. 7 ·1994-04-00 ·Pages 1957-68

Chistoserdova LV, Lidstrom ME

Abstract

In a previous paper, we reported identification of the 5' part of hprA of Methylobacterium extorquens AM1, which encodes the serine cycle enzyme hydroxypyruvate reductase (L. V. Chistoserdova and M. E. Lidstrom, J. Bacteriol. 174:71-77, 1992). Here we present the complete sequence of hprA and partial sequence of genes adjacent to hprA. Upstream of hprA, the 3' part of an open reading frame was discovered, separated from hprA by 263 bp. This open reading frame was identified as the gene encoding another serine cycle enzyme, serine glyoxylate aminotransferase (sgaA). Cells containing an insertion mutation into sgaA were unable to grow on C1 compounds, demonstrating that the gene is required for C1 metabolism. Sequencing downstream of hprA has revealed the presence of another open reading frame (mtdA), which is probably cotranscribed with hprA. This open reading frame was identified as the gene required for the synthesis of 5,10-methylenetetrahydrofolate dehydrogenase. Our data suggest that this enzyme plays an integral role in methylotrophic metabolism in M. extorquens AM1, either in formaldehyde oxidation or as part of the serine cycle.

Related Genes
hprA mtdA sgA
MeSH Terms
Alcohol Oxidoreductases/genetics Amino Acid Sequence Base Sequence DNA Mutational Analysis Gene Expression Gram-Negative Anaerobic Bacteria/genetics Hydroxypyruvate Reductase Methylenetetrahydrofolate Dehydrogenase (NADP)/biosynthesis,genetics Molecular Sequence Data Mutagenesis, Insertional Open Reading Frames/genetics Recombinant Proteins/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Serine/metabolism Transaminases/genetics
Chemicals
Recombinant Proteins Serine Alcohol Oxidoreductases Hydroxypyruvate Reductase Methylenetetrahydrofolate Dehydrogenase (NADP) Transaminases glyoxylate aminotransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chistoserdova L V
W. M. Keck Laboratories 138-78, California Institute of Technology, Pasadena 91125.
Lidstrom M E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.7.1957-1968.1994
Published
1994-04-00
Pages
1957-68
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205300
Subset
IM
Grants
NIGMS NIH HHS · GM36294 · United States
Databases
GENBANK
L27235
External Links
PubMed source DOI: 10.1128/jb.176.7.1957-1968.1994 PMC Full Text View journal details
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