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PMID: 8246987 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphatidylinositol (PI) 3-kinase and PI 4-kinase binding to the CD4-p56lck complex: the p56lck SH3 domain binds to PI 3-kinase but not PI 4-kinase.

Molecular and cellular biology ·Vol. 13 ·No. 12 ·1993-12-00 ·Pages 7708-17

Prasad KV, Kapeller R, Janssen O, Repke H, Duke-Cohan JS, Cantley LC, Rudd CE

Abstract

CD4 serves as a receptor for major histocompatibility complex class II antigens and as a receptor for the human immunodeficiency virus type 1 (HIV-1) viral coat protein gp120. It is coupled to the protein-tyrosine kinase p56lck, an interaction necessary for an optimal response of certain T cells to antigen. In addition to the protein-tyrosine kinase domain, p56lck possesses Src homology 2 and 3 (SH2 and SH3) domains as well as a unique N-terminal region. The mechanism by which p56lck generates intracellular signals is unclear, although it has the potential to interact with various downstream molecules. One such downstream target is the lipid kinase phosphatidylinositol 3-kinase (PI 3-kinase), which has been found to bind to activated pp60src and receptor-tyrosine kinases. In this study, we verified that PI 3-kinase associates with the CD4:p56lck complex as judged by the presence of PI 3-phosphate generated from anti-CD4 immunoprecipitates and detected by high-pressure liquid chromatographic analysis. However, surprisingly, CD4-p56lck was also found to associate with another lipid kinase, phosphatidylinositol 4-kinase (PI 4-kinase). The level of associated PI 4-kinase was generally higher than PI 3-kinase activity. HIV-1 gp120 and antibody-mediated cross-linking induced a 5- to 10-fold increase in the level of CD4-associated PI 4- and PI 3-kinases. The use of glutathione S-transferase fusion proteins carrying Lck-SH2, Lck-SH3, and Lck-SH2/SH3 domains showed PI 3-kinase binding to the SH3 domain of p56lck, an interaction facilitated by the presence of an adjacent SH2 domain. PI 4-kinase bound to neither the SH2 nor the SH3 domain of p56lck. CD4-p56lck contributes PI 3- and PI 4-kinase to the activation process of T cells and may play a role in HIV-1-induced immune defects.

MeSH Terms
1-Phosphatidylinositol 4-Kinase Amino Acid Sequence Binding Sites CD4 Antigens/genetics,metabolism Cell Line HIV Envelope Protein gp120/metabolism Humans Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Molecular Sequence Data Phosphatidylinositol 3-Kinases Phosphotransferases (Alcohol Group Acceptor)/metabolism Protein Binding Protein-Tyrosine Kinases/chemistry,genetics,metabolism Receptors, HIV/metabolism T-Lymphocytes/metabolism
Chemicals
CD4 Antigens HIV Envelope Protein gp120 Receptors, HIV Phosphatidylinositol 3-Kinases Phosphotransferases (Alcohol Group Acceptor) 1-Phosphatidylinositol 4-Kinase Protein-Tyrosine Kinases Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Prasad K V
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Kapeller R
Janssen O
Repke H
Duke-Cohan J S
Cantley L C
Rudd C E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.12.7708-7717.1993
Published
1993-12-00
Pages
7708-17
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364842
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041890 · United States
NIGMS NIH HHS · GM 36624 · United States
NIGMS NIH HHS · GM 41890 · United States
NCI NIH HHS · NCI CA51887-02 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.12.7708-7717.1993 PMC Full Text View journal details
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