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PMID: 8248184 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Gastric DNA-binding proteins recognize upstream sequence motifs of parietal cell-specific genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 22 ·1993-11-15 ·Pages 10876-80

Tamura S, Wang XH, Maeda M, Futai M

Abstract

Polymerase chain reaction amplification of cDNA from pig gastric mucosa demonstrated the presence of zinc-finger proteins called GATA-GT1, GATA-GT2, and GATA-GT3, each having zinc-finger sequences similar to previously characterized GATA-binding proteins. Subsequently, full-length cDNAs of GATA-GT1 and GATA-GT2 were obtained from rat stomach. The zinc-finger domains of GATA-GT1 and -GT2 were 66-86% identical on the amino acid level with each other and with other GATA-binding proteins. Potential protein kinase phosphorylation sites were present in the zinc-finger region. In contrast, regions outside the zinc fingers shared significantly lower similarities. GATA-GT2 was found to bind to the upstream sequence of the H+/K(+)-ATPase beta gene and to a sequence containing the GATA motif. GATA-GT1 and -GT2 were expressed predominantly in the gastric mucosa and at much lower levels in the intestine (GATA-GT2, also in testis), their tissue distributions being distinct from those of GATA-1, -2, or -3. These results clearly suggest that GATA-GT1 and GATA-GT2 are involved in gene regulation specifically in the gastric epithelium and represent two additional members of the GATA-binding protein family.

Related Genes
GATA-1 GATA-2 GATA-3 GATA-GT1 GATA-GT2 GATA-GT3
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Primers/chemistry DNA, Complementary/genetics DNA-Binding Proteins/genetics GATA4 Transcription Factor GATA6 Transcription Factor Gastric Mucosa/physiology Gene Expression Molecular Sequence Data Multigene Family Polymerase Chain Reaction RNA, Messenger/genetics Rats Rats, Sprague-Dawley Sequence Alignment Sequence Homology, Amino Acid Swine Tissue Distribution Transcription Factors/genetics Zinc Fingers
Chemicals
DNA Primers DNA, Complementary DNA-Binding Proteins GATA4 Transcription Factor GATA6 Transcription Factor Gata4 protein, rat Gata6 protein, rat RNA, Messenger Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tamura S
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
Wang X H
Maeda M
Futai M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.22.10876
Published
1993-11-15
Pages
10876-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47881
Subset
IM
Databases
GENBANK
L22760, L22761
Corrections
ErratumIn
PMID: 8183957
External Links
PubMed source DOI: 10.1073/pnas.90.22.10876 PMC Full Text View journal details
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