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PMID: 8455608 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart.

Molecular and cellular biology ·Vol. 13 ·No. 4 ·1993-04-00 ·Pages 2235-46

Arceci RJ, King AA, Simon MC, Orkin SH, Wilson DB

Abstract

We report the cDNA cloning and characterization of mouse GATA-4, a new member of the family of zinc finger transcription factors that bind a core GATA motif. GATA-4 cDNA was identified by screening a 6.5-day mouse embryo library with oligonucleotide probes corresponding to a highly conserved region of the finger domains. Like other proteins of the family, GATA-4 is approximately 50 kDa in size and contains two zinc finger domains of the form C-X-N-C-(X17)-C-N-X-C. Cotransfection assays in heterologous cells demonstrate that GATA-4 trans activates reporter constructs containing GATA promoter elements. Northern (RNA) analysis and in situ hybridization show that GATA-4 mRNA is expressed in the heart, intestinal epithelium, primitive endoderm, and gonads. Retinoic acid-induced differentiation of mouse F9 cells into visceral or parietal endoderm is accompanied by increased expression of GATA-4 mRNA and protein. In vitro differentiation of embryonic stem cells into embryoid bodies is also associated with increased GATA-4 expression. We conclude that GATA-4 is a tissue-specific, retinoic acid-inducible, and developmentally regulated transcription factor. On the basis of its tissue distribution, we speculate that GATA-4 plays a role in gene expression in the heart, intestinal epithelium, primitive endoderm, and gonads.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Differentiation Cloning, Molecular Consensus Sequence DNA-Binding Proteins/genetics GATA4 Transcription Factor Gene Expression Regulation Genes In Situ Hybridization Mice Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger/genetics Sequence Alignment Sequence Deletion Structure-Activity Relationship Transcription Factors/genetics Transcriptional Activation Tretinoin/pharmacology Zinc Fingers
Chemicals
DNA-Binding Proteins GATA4 Transcription Factor RNA, Messenger Transcription Factors Tretinoin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Arceci R J
Division of Pediatric Hematology-Oncology, Boston Children's Hospital, Massachusetts.
King A A
Simon M C
Orkin S H
Wilson D B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.4.2235-2246.1993
Published
1993-04-00
Pages
2235-46
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359544
Subset
IM
Databases
GENBANK
M98339
External Links
PubMed source DOI: 10.1128/mcb.13.4.2235-2246.1993 PMC Full Text View journal details
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